"Blotting, Southwestern" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus,
MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure,
which enables searching at various levels of specificity.
A method that is used to detect DNA-protein interactions. Proteins are separated by electrophoresis and blotted onto a nitrocellulose membrane similar to Western blotting (BLOTTING, WESTERN) but the proteins are identified when they bind labeled DNA PROBES (as with Southern blotting (BLOTTING, SOUTHERN)) instead of antibodies.
| Descriptor ID |
D026501
|
| MeSH Number(s) |
E05.196.401.133 E05.301.300.093 E05.601.225
|
| Concept/Terms |
Blotting, Southwestern- Blotting, Southwestern
- Blottings, Southwestern
- Southwestern Blottings
- Southwestern Blotting
Blot, Southwestern- Blot, Southwestern
- Blots, Southwestern
- Southwestern Blots
- Southwestern Blot
|
Below are MeSH descriptors whose meaning is more general than "Blotting, Southwestern".
Below are MeSH descriptors whose meaning is more specific than "Blotting, Southwestern".
This graph shows the total number of publications written about "Blotting, Southwestern" by people in this website by year, and whether "Blotting, Southwestern" was a major or minor topic of these publications.
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| Year | Major Topic | Minor Topic | Total |
|---|
| 2009 | 1 | 1 | 2 |
| 2010 | 1 | 0 | 1 |
| 2011 | 1 | 0 | 1 |
| 2012 | 0 | 2 | 2 |
| 2015 | 1 | 0 | 1 |
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Below are the most recent publications written about "Blotting, Southwestern" by people in Profiles.
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Jia Y, Nagore L, Jarrett H. Southwestern Blotting Assay. Methods Mol Biol. 2015; 1334:85-99.
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Zhou Y, Jia Y, Jarrett HW. Asymmetric polymerase chain reaction provides alternatives for preparation of (GT)5-tailed duplex DNA promoter for promoter trapping. Anal Biochem. 2012 Aug 15; 427(2):133-8.
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Jarrett HW. Proteomic methodologies to study transcription factor function. Methods Mol Biol. 2012; 786:315-34.
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Jiang D, Jia Y, Jarrett HW. Transcription factor proteomics: identification by a novel gel mobility shift-three-dimensional electrophoresis method coupled with southwestern blot and high-performance liquid chromatography-electrospray-mass spectrometry analysis. J Chromatogr A. 2011 Sep 28; 1218(39):7003-15.
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Jia Y, Jiang D, Jarrett HW. Repeated probing of Southwestern blots using alkaline phosphatase stripping. J Chromatogr A. 2010 Nov 05; 1217(45):7177-81.
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Jiang D, Jarrett HW, Haskins WE. Methods for proteomic analysis of transcription factors. J Chromatogr A. 2009 Oct 09; 1216(41):6881-9.
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Jiang D, Jia Y, Zhou Y, Jarrett HW. Two-dimensional southwestern blotting and characterization of transcription factors on-blot. J Proteome Res. 2009 Jul; 8(7):3693-701.