Two-Hybrid System Techniques
"Two-Hybrid System Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus,
MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure,
which enables searching at various levels of specificity.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
| Descriptor ID |
D020798
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| MeSH Number(s) |
E05.393.220.870 E05.601.690.650 E05.601.870
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| Concept/Terms |
Two-Hybrid System Techniques- Two-Hybrid System Techniques
- Technique, Two-Hybrid System
- Techniques, Two-Hybrid System
- Two Hybrid System Techniques
- Two-Hybrid System Technique
- Two-Hybrid Method
- Method, Two-Hybrid
- Methods, Two-Hybrid
- Two Hybrid Method
- Two-Hybrid Methods
- Two-Hybrid System Technics
- Technic, Two-Hybrid System
- Technics, Two-Hybrid System
- Two Hybrid System Technics
- Two-Hybrid System Technic
- Two-Hybrid Assay
- Assay, Two-Hybrid
- Assays, Two-Hybrid
- Two Hybrid Assay
- Two-Hybrid Assays
One-Hybrid System Techniques- One-Hybrid System Techniques
- One Hybrid System Techniques
- One-Hybrid System Technique
- Technique, One-Hybrid System
- Techniques, One-Hybrid System
- One-Hybrid System Technics
- One Hybrid System Technics
- One-Hybrid System Technic
- Technic, One-Hybrid System
- Technics, One-Hybrid System
Yeast Two-Hybrid System Techniques- Yeast Two-Hybrid System Techniques
- Yeast Two Hybrid System Techniques
- Yeast Two-Hybrid System
- System, Yeast Two-Hybrid
- Systems, Yeast Two-Hybrid
- Two-Hybrid System, Yeast
- Two-Hybrid Systems, Yeast
- Yeast Two Hybrid System
- Yeast Two-Hybrid Systems
- Yeast Two-Hybrid Assay
- Assay, Yeast Two-Hybrid
- Assays, Yeast Two-Hybrid
- Two-Hybrid Assay, Yeast
- Two-Hybrid Assays, Yeast
- Yeast Two Hybrid Assay
- Yeast Two-Hybrid Assays
Three-Hybrid System Techniques- Three-Hybrid System Techniques
- Technique, Three-Hybrid System
- Techniques, Three-Hybrid System
- Three Hybrid System Techniques
- Three-Hybrid System Technique
- Tri-Hybrid System Techniques
- Technique, Tri-Hybrid System
- Techniques, Tri-Hybrid System
- Tri Hybrid System Techniques
- Tri-Hybrid System Technique
- Three-Hybrid System Technics
- Technic, Three-Hybrid System
- Technics, Three-Hybrid System
- Three Hybrid System Technics
- Three-Hybrid System Technic
Yeast Three-Hybrid System Techniques- Yeast Three-Hybrid System Techniques
- Yeast Three Hybrid System Techniques
- Yeast Three-Hybrid System
- System, Yeast Three-Hybrid
- Systems, Yeast Three-Hybrid
- Three-Hybrid System, Yeast
- Three-Hybrid Systems, Yeast
- Yeast Three Hybrid System
- Yeast Three-Hybrid Systems
- Yeast Three-Hybrid Assay
- Assay, Yeast Three-Hybrid
- Assays, Yeast Three-Hybrid
- Three-Hybrid Assay, Yeast
- Three-Hybrid Assays, Yeast
- Yeast Three Hybrid Assay
- Yeast Three-Hybrid Assays
n-Hybrid System Techniques- n-Hybrid System Techniques
- System Technique, n-Hybrid
- System Techniques, n-Hybrid
- Technique, n-Hybrid System
- Techniques, n-Hybrid System
- n Hybrid System Techniques
- n-Hybrid System Technique
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Below are MeSH descriptors whose meaning is more general than "Two-Hybrid System Techniques".
Below are MeSH descriptors whose meaning is more specific than "Two-Hybrid System Techniques".
This graph shows the total number of publications written about "Two-Hybrid System Techniques" by people in this website by year, and whether "Two-Hybrid System Techniques" was a major or minor topic of these publications.
To see the data from this visualization as text,
click here.
| Year | Major Topic | Minor Topic | Total |
|---|
| 2000 | 0 | 2 | 2 |
| 2001 | 0 | 2 | 2 |
| 2002 | 0 | 2 | 2 |
| 2003 | 0 | 3 | 3 |
| 2004 | 0 | 4 | 4 |
| 2005 | 0 | 5 | 5 |
| 2006 | 0 | 2 | 2 |
| 2007 | 0 | 5 | 5 |
| 2008 | 0 | 1 | 1 |
| 2010 | 1 | 2 | 3 |
| 2011 | 0 | 4 | 4 |
| 2014 | 0 | 4 | 4 |
| 2015 | 0 | 3 | 3 |
| 2016 | 0 | 2 | 2 |
| 2018 | 0 | 1 | 1 |
| 2020 | 0 | 1 | 1 |
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Below are the most recent publications written about "Two-Hybrid System Techniques" by people in Profiles.
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Whitmore A, Freeny D, Sojourner SJ, Miles JS, Graham WM, Flores-Rozas H. Evaluation of the Role of Human DNAJAs in the Response to Cytotoxic Chemotherapeutic Agents in a Yeast Model System. Biomed Res Int. 2020; 2020:9097638.
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Smith JT, Singha UK, Misra S, Chaudhuri M. Divergent Small Tim Homologues Are Associated with TbTim17 and Critical for the Biogenesis of TbTim17 Protein Complexes in Trypanosoma brucei. mSphere. 2018 06 27; 3(3).
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Santiago E, Akamine P, Snider J, Wong V, Jessulat M, Deineko V, Gagarinova A, Aoki H, Minic Z, Phanse S, San Antonio A, Cubano LA, Rymond BC, Babu M, Stagljar I, Rodriguez-Medina JR. Novel Interactome of Saccharomyces cerevisiae Myosin Type?II Identified by a Modified Integrated Membrane Yeast Two-Hybrid (iMYTH) Screen. G3 (Bethesda). 2016 05 03; 6(5):1469-74.
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Huang KH, Mychack A, Tchorzewski L, Janakiraman A. Characterization of the FtsZ C-Terminal Variable (CTV) Region in Z-Ring Assembly and Interaction with the Z-Ring Stabilizer ZapD in E. coli Cytokinesis. PLoS One. 2016; 11(4):e0153337.
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Schumacher MA, Zeng W, Huang KH, Tchorzewski L, Janakiraman A. Structural and Functional Analyses Reveal Insights into the Molecular Properties of the Escherichia coli Z Ring Stabilizing Protein, ZapC. J Biol Chem. 2016 Jan 29; 291(5):2485-98.
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Ravindranath AK, Kaur S, Wernyj RP, Kumaran MN, Miletti-Gonzalez KE, Chan R, Lim E, Madura K, Rodriguez-Rodriguez L. CD44 promotes multi-drug resistance by protecting P-glycoprotein from FBXO21-mediated ubiquitination. Oncotarget. 2015 Sep 22; 6(28):26308-21.
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Losh JS, King AK, Bakelar J, Taylor L, Loomis J, Rosenzweig JA, Johnson SJ, van Hoof A. Interaction between the RNA-dependent ATPase and poly(A) polymerase subunits of the TRAMP complex is mediated by short peptides and important for snoRNA processing. Nucleic Acids Res. 2015 Feb 18; 43(3):1848-58.
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Balasubramaniam M, Kim BS, Hutchens-Williams HM, Loesch-Fries LS. The photosystem II oxygen-evolving complex protein PsbP interacts with the coat protein of Alfalfa mosaic virus and inhibits virus replication. Mol Plant Microbe Interact. 2014 Oct; 27(10):1107-18.
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Pillai JK, Venkadesh S, Ajees AA, Rosen BP, Bhattacharjee H. Mutations in the ArsA ATPase that restore interaction with the ArsD metallochaperone. Biometals. 2014 Dec; 27(6):1263-75.
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Nguyen JQ, Gilley RP, Zogaj X, Rodriguez SA, Klose KE. Lipidation of the FPI protein IglE contributes to Francisella tularensis ssp. novicida intramacrophage replication and virulence. Pathog Dis. 2014 Oct; 72(1):10-8.