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Michael D. Powell
Title Associate Professor, Dept. of MBI
Faculty Rank
Degree Ph.D.
Institution Morehouse School of Medicine
Department Microbiology, Biochemistry and Immunology
Clusters HIV/AIDS
Address
720 Westview Dr. SW
City Atlanta
State GA
Postal Code 30310
Telephone 404-752-1582
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  My research centers around the HIV-1 protein Nef. Our research has shown that Nef protein is secreted by HIV-1 infected cells and is present in the plasma of infected individuals. Our studies suggest that Nef is secreted from cells in the form of exosomes, small membrane bound vesicles approximately 50-100nm in size. T cells that come in contact with Nef exosomes undego apoptosis. Macrophage can take up Nef exosomes, but their affect on these cells is largely unknown. Nef exosomes appear to play a significant role in the pathogenic effects of HIV-1 infection leading to AIDS. Most recently we have worked with Bruce Shiramizu at the U of Hawaii to investigate the potential of Nef exosomes to facilitate the pathogenesis of HIV Associated Neurologic Disorders (HAND).

We have also been working with a traditional medicine group called ProMetra. Extracts of natural plants are used to treat HIV/AIDS patients. We have shown that these natural products have the ability to block entry of HIV into T cells. We are interested testing these herbal treatments in a clinical trial. We have recently worked with USAMRIID to also investigate the potential of these herbal treatments to inhibit Ebola virus.

In another collaboration we are working with Carol Carter at SUNY Stonybrook to investigate host proteins involved in trafficking of Gag protein in HIV-1. Our most recent work has been investigating the host protein "Sprouty" in viral traffiicking,

I am also the director of our local Proteomics facility we perform LC/MS protein identification and Luminex multiplex protein anaysis.. I would be happy to discuss potential projects with anyone looking for a collaborator.

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1. Anyanwu SI, Doherty A, Powell MD, Obialo C, Huang MB, Quarshie A, Mitchell C, Bashir K, Newman GW. Detection of HIV-1 and Human Proteins in Urinary Extracellular Vesicles from HIV+ Patients. Adv Virol. 2018; 2018:7863412.
2. Strickland M, Ehrlich LS, Watanabe S, Khan M, Strub MP, Luan CH, Powell MD, Leis J, Tjandra N, Carter CA. Tsg101 chaperone function revealed by HIV-1 assembly inhibitors. Nat Commun. 2017 11 09; 8(1):1391.
3. Konadu KA, Huang MB, Roth W, Armstrong W, Powell M, Villinger F, Bond V. Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals. J Vis Exp. 2016 Jan 05; (107).
4. Huang MB, Ye L, Liang BY, Ning CY, Roth WW, Jiang JJ, Huang JG, Zhou B, Zang N, Powell MD, Liang H, Bond VC. Characterizing the HIV/AIDS Epidemic in the United States and China. Int J Environ Res Public Health. 2015 Dec 22; 13(1):ijerph13010030.
5. Roth WW, Huang MB, Addae Konadu K, Powell MD, Bond VC. Micro RNA in Exosomes from HIV-Infected Macrophages. Int J Environ Res Public Health. 2015 Dec 22; 13(1):ijerph13010032.
6. Khan MB, Lang MJ, Huang MB, Raymond A, Bond VC, Shiramizu B, Powell MD. Nef exosomes isolated from the plasma of individuals with HIV-associated dementia (HAD) can induce Aß(1-42) secretion in SH-SY5Y neural cells. J Neurovirol. 2016 Apr; 22(2):179-90.
7. Konadu KA, Anderson JS, Huang MB, Ali SA, Powell MD, Villinger F, Bond VC. Hallmarks of HIV-1 pathogenesis are modulated by Nef''s Secretion Modification Region. J AIDS Clin Res. 2015 Jul; 6(7).
8. Konadu KA, Chu J, Huang MB, Amancha PK, Armstrong W, Powell MD, Villinger F, Bond VC. Association of Cytokines With Exosomes in the Plasma of HIV-1-Seropositive Individuals. J Infect Dis. 2015 Jun 01; 211(11):1712-6.
9. Singh A, Powell MD, Sridaran R, Krishna A. Effects of seasonal adiposity on ovarian activity of Vespertilionid bat, Scotophilus heathi: proteomics analysis. Mol Cell Endocrinol. 2015 Jan 05; 399:219-27.
10. Shelton MN, Huang MB, Ali S, Johnson K, Roth W, Powell M, Bond V. Peptide-based identification of functional motifs and their binding partners. J Vis Exp. 2013 Jun 30; (76).
11. Campbell PE, Isayev O, Ali SA, Roth WW, Huang MB, Powell MD, Leszczynski J, Bond VC. Validation of a novel secretion modification region (SMR) of HIV-1 Nef using cohort sequence analysis and molecular modeling. J Mol Model. 2012 Oct; 18(10):4603-13.
12. Omole FS, Lin JS, Chu T, Sow CM, Flood A, Powell MD. Raynaud''s phenomenon, cytokines and acupuncture: a case report. Acupunct Med. 2012 Jun; 30(2):139-41.
13. Shelton MN, Huang MB, Ali SA, Powell MD, Bond VC. Secretion modification region-derived peptide disrupts HIV-1 Nef''s interaction with mortalin and blocks virus and Nef exosome release. J Virol. 2012 Jan; 86(1):406-19.
14. Zimmerman SW, Manandhar G, Yi YJ, Gupta SK, Sutovsky M, Odhiambo JF, Powell MD, Miller DJ, Sutovsky P. Sperm proteasomes degrade sperm receptor on the egg zona pellucida during mammalian fertilization. PLoS One. 2011 Feb 23; 6(2):e17256.
15. Ehrlich LS, Medina GN, Khan MB, Powell MD, Mikoshiba K, Carter CA. Activation of the inositol (1,4,5)-triphosphate calcium gate receptor is required for HIV-1 Gag release. J Virol. 2010 Jul; 84(13):6438-51.
16. Ali SA, Huang MB, Campbell PE, Roth WW, Campbell T, Khan M, Newman G, Villinger F, Powell MD, Bond VC. Genetic characterization of HIV type 1 Nef-induced vesicle secretion. AIDS Res Hum Retroviruses. 2010 Feb; 26(2):173-92.
17. Powell MD, Manandhar G, Spate L, Sutovsky M, Zimmerman S, Sachdev SC, Hannink M, Prather RS, Sutovsky P. Discovery of putative oocyte quality markers by comparative ExacTag proteomics. Proteomics Clin Appl. 2010 Mar; 4(3):337-51.
18. Harrington W, Bond V, Huang MB, Powell M, Lillard J, Manne U, Bumpers H. HIV Nef-M1 Effects on Colorectal Cancer Growth in Tumor-induced Spleens and Hepatic Metastasis. Mol Cell Pharmacol. 2009 Aug 05; 1(2):85-91.
19. Wilson NO, Huang MB, Anderson W, Bond V, Powell M, Thompson WE, Armah HB, Adjei AA, Gyasi R, Tettey Y, Stiles JK. Soluble factors from Plasmodium falciparum-infected erythrocytes induce apoptosis in human brain vascular endothelial and neuroglia cells. Mol Biochem Parasitol. 2008 Dec; 162(2):172-6.
20. Wang F, Xia P, Wu F, Wang D, Wang W, Ward T, Liu Y, Aikhionbare F, Guo Z, Powell M, Liu B, Bi F, Shaw A, Zhu Z, Elmoselhi A, Fan D, Cover TL, Ding X, Yao X. Helicobacter pylori VacA disrupts apical membrane-cytoskeletal interactions in gastric parietal cells. J Biol Chem. 2008 Sep 26; 283(39):26714-25.
21. Campbell TD, Khan M, Huang MB, Bond VC, Powell MD. HIV-1 Nef protein is secreted into vesicles that can fuse with target cells and virions. Ethn Dis. 2008; 18(2 Suppl 2):S2-14-9.
22. Huang MB, James CO, Powell MD, Bond VC. Apoptotic peptides derived from HIV-1 Nef induce lymphocyte depletion in mice. Ethn Dis. 2008; 18(2 Suppl 2):S2-30-7.
23. Armah HB, Wilson NO, Sarfo BY, Powell MD, Bond VC, Anderson W, Adjei AA, Gyasi RK, Tettey Y, Wiredu EK, Tongren JE, Udhayakumar V, Stiles JK. Cerebrospinal fluid and serum biomarkers of cerebral malaria mortality in Ghanaian children. Malar J. 2007 Nov 12; 6:147.
24. Thomas K, Wu J, Sung DY, Thompson W, Powell M, McCarrey J, Gibbs R, Walker W. SP1 transcription factors in male germ cell development and differentiation. Mol Cell Endocrinol. 2007 May 30; 270(1-2):1-7.
25. Miles LR, Agresta BE, Khan MB, Tang S, Levin JG, Powell MD. Effect of polypurine tract (PPT) mutations on human immunodeficiency virus type 1 replication: a virus with a completely randomized PPT retains low infectivity. J Virol. 2005 Jun; 79(11):6859-67.
26. Khan M, Jin L, Miles L, Bond VC, Powell MD. Chimeric human immunodeficiency virus type 1 virions that contain the simian immunodeficiency virus nef gene are cyclosporin A resistant. J Virol. 2005 Mar; 79(5):3211-6.
27. Bumpers HL, Huang MB, Powell M, Grizzle WE, Lillard JW, Okoli J, Bond VC. Effects of HIV-1 Nef, a cytotoxic viral protein, on the growth of primary colorectal cancer. Cancer Biol Ther. 2005 Jan; 4(1):65-9.
28. Huang MB, Jin LL, James CO, Khan M, Powell MD, Bond VC. Characterization of Nef-CXCR4 interactions important for apoptosis induction. J Virol. 2004 Oct; 78(20):11084-96.
29. James CO, Huang MB, Khan M, Garcia-Barrio M, Powell MD, Bond VC. Extracellular Nef protein targets CD4+ T cells for apoptosis by interacting with CXCR4 surface receptors. J Virol. 2004 Mar; 78(6):3099-109.
30. Stiles JK, Whittaker J, Sarfo BY, Thompson WE, Powell MD, Bond VC. Trypanosome apoptotic factor mediates apoptosis in human brain vascular endothelial cells. Mol Biochem Parasitol. 2004 Feb; 133(2):229-40.
31. Khan M, Jin L, Huang MB, Miles L, Bond VC, Powell MD. Chimeric human immunodeficiency virus type 1 (HIV-1) virions containing HIV-2 or simian immunodeficiency virus Nef are resistant to cyclosporine treatment. J Virol. 2004 Feb; 78(4):1843-50.
32. Post K, Guo J, Howard KJ, Powell MD, Miller JT, Hizi A, Le Grice SF, Levin JG. Human immunodeficiency virus type 2 reverse transcriptase activity in model systems that mimic steps in reverse transcription. J Virol. 2003 Jul; 77(13):7623-34.
33. Khan M, Garcia-Barrio M, Powell MD. Treatment of human immunodeficiency virus type 1 virions depleted of cyclophilin A by natural endogenous reverse transcription restores infectivity. J Virol. 2003 Apr; 77(7):4431-4.
34. Khan M, Garcia-Barrio M, Powell MD. Restoration of wild-type infectivity to human immunodeficiency virus type 1 strains lacking nef by intravirion reverse transcription. J Virol. 2001 Dec; 75(24):12081-7.
35. Huang MB, Khan M, Garcia-Barrio M, Powell M, Bond VC. Apoptotic effects in primary human umbilical vein endothelial cell cultures caused by exposure to virion-associated and cell membrane-associated HIV-1 gp120. J Acquir Immune Defic Syndr. 2001 Jul 01; 27(3):213-21.

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HIV-1
nef Gene Products, Human Immunodeficiency Virus
Exosomes
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