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Hong Yang
Title Dr.
Faculty Rank Professor
Degree M.D.
Institution Meharry Medical College
Department Physiology
Clusters Cardiovascular and Related Diseases
Address
1005 Dr. D.B. Todd
City nashville
State TN
Postal Code 37208-350
Telephone 615.327.5772
Fax
Email
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Awards and Honors icon

2003 AFAR Recipient AFAR A03109 award to study the age-related increase in the sensitivity of endothelial cell
2004 NIH Recipient K01HL0076623 award to study the role of oxidative stress in endothelial aging.
2010 NIH NIH SC1 HL101431: Defective isoforms of apoE induce atherogenesis via unfolded protein responses
2010 Recipient U54RR026140 pilot award to study the role of suppression of unfolded protein response in f
2012 SERLC Travel award for oral presentation

Narrative icon

  I am an enthusiastic researcher with specific training and expertise in atherosclerosis pathology, lipid metabolism and molecular biology. Since being appointed as a faculty member in Meharry Medical College 12 years ago, I have been successful in acquiring research funding from the National Institutes of Health and the American Federation for Aging Research (AFAR). Our laboratory has developed a number of techniques in studying the pathogenesis of atherosclerosis in vitro and in vivo. Specifically: a) we have developed methods to assess the histology and immunohistochemistry changes in mouse tissues; b) refined molecular tools for quantifying the steady-state levels of proteins in these target cells and tissues; c) refined methods for assessing not only the steady state levels of mRNAs but also the efficiency mRNA transcription and translation in tissues and cells; and d) developed a proteomics laboratory, which is equipped with 2-D electrophoresis systems, protein spot picker, Typhoon laser scanner and MALDI-TOF-mass spectrometry. With this system, we examined the changes induced by apoE-deficient lipoproteins, atherosclerosis and hypertension in protein profile in cultured cells, animal tissues and human blood. To test many hypotheses at the cellular level, our laboratory has developed protocols for the successful culturing of mouse endothelial cells, smooth muscle cells and peritoneal macrophages that overexpress or underexpress various genes. To study the impact of various interventions on atherosclerosis in vivo, we developed mouse models by crossbreeding existing transgenic and knockout mice. An example is the mouse model that lacks apoE and overexpresses antioxidant enzyme GPx4. Therefore we expect the studies in this proposal to proceed without any technical difficulties.
More than this, I have established working relationships with several faculties at Vanderbilt (which is ~ 2 miles from Meharry). As background, in spring, 1999, Meharry and Vanderbilt co-signed an MOU forming the Meharry-Vanderbilt Alliance. Since that time, we have worked together in areas of mutual interest including research, patient care and education. As part of this agreement, our libraries and research cores are open for use by faculty, students on both campuses and a number of faculty hold adjunct appts at the other institution. Thus since ~2005, I have enjoyed a cordial working relationship with faculty at Vanderbilt U Medical Ctr such as L.Jackson Roberts, MD (discoverer of isoprostanes who studies free-radical damage/oxidative stress) and Sergio Fazio, MD, PhD and MacRae Linton, MD, experts in dyslipidemia and atherosclerosis. Although Dr. Fazio recently moved to the West Coast, I frequently contact Drs. Roberts and Linton advice. I also collaborated with several cardiovascular research and other experts, including Drs. Shawn Goodwin and ZhongMao Guo at Meharry and Arlan Richardson at UT-San Antonio. Finally, the research core facilities at Meharry are superb in supporting our proposed research, including the molecular biology core, the confocal morphology core, and the tissue preparation for histology/ immunohistochemistry core. Thus, all of the essential equipment and expertise needed for our proposed work is readily available in this research environment.

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1. Yang F, Yang H, Ramesh A, Goodwin JS, Okoro EU, Guo Z. Overexpression of Catalase Enhances Benzo(a)pyrene Detoxification in Endothelial Microsomes. PLoS One. 2016; 11(9):e0162561.
2. Okoro EU, Guo Z, Yang H. Akt isoform-dependent regulation of ATP-Binding cassette A1 expression by apolipoprotein E. Biochem Biophys Res Commun. 2016 08 12; 477(1):123-8.
3. Okoro EU, Zhang H, Guo Z, Yang F, Smith C, Yang H. A Subregion of Reelin Suppresses Lipoprotein-Induced Cholesterol Accumulation in Macrophages. PLoS One. 2015; 10(8):e0136895.
4. Miao L, Okoro EU, Cao Z, Yang H, Motley-Johnson E, Guo Z. High-density lipoprotein-mediated transcellular cholesterol transport in mouse aortic endothelial cells. Biochem Biophys Res Commun. 2015 Sep 18; 465(2):256-61.
5. Shan G, Yang F, Zhou L, Tang T, Okoro EU, Yang H, Guo Z. Increase in Blood Glutathione and Erythrocyte Proteins Related to Glutathione Generation, Reduction and Utilization in African-American Old Women with Diabetes. J Sci Technol Environ. 2015; 5(1).
6. Zhou L, Yang H, Okoro EU, Guo Z. Up-regulation of cholesterol absorption is a mechanism for cholecystokinin-induced hypercholesterolemia. J Biol Chem. 2014 May 09; 289(19):12989-99.
7. Wu J, Xiao Y, Liu J, Yang H, Dong X, Hu S, Jin S, Wu D. Potential role of ATM in hepatocyte endocytosis of ApoE-deficient, ApoB48-containing lipoprotein in ApoE-deficient mice. Int J Mol Med. 2014 Feb; 33(2):462-8.
8. Zhou L, Yang D, Wu DF, Guo ZM, Okoro E, Yang H. Inhibition of endoplasmic reticulum stress and atherosclerosis by 2-aminopurine in apolipoprotein e-deficient mice. ISRN Pharmacol. 2013; 2013:847310.
9. Lin X, Yang H, Zhang H, Zhou L, Guo Z. A novel transcription mechanism activated by ethanol: induction of Slc7a11 gene expression via inhibition of the DNA-binding activity of transcriptional repressor octamer-binding transcription factor 1 (OCT-1). J Biol Chem. 2013 May 24; 288(21):14815-23.
10. Zhou L, Yang H, Lin X, Okoro EU, Guo Z. Cholecystokinin elevates mouse plasma lipids. PLoS One. 2012; 7(12):e51011.
11. Okoro EU, Zhao Y, Guo Z, Zhou L, Lin X, Yang H. Apolipoprotein E4 is deficient in inducing macrophage ABCA1 expression and stimulating the Sp1 signaling pathway. PLoS One. 2012; 7(9):e44430.
12. Ravella K, Yang H, Gangula PR. Impairment of gastric nitrergic and NRF2 system in apolipoprotein E knockout mice. Dig Dis Sci. 2012 Jun; 57(6):1504-9.
13. Zhang HF, Lin XH, Yang H, Zhou LC, Guo YL, Barnett JV, Guo ZM. Regulation of the activity and expression of aryl hydrocarbon receptor by ethanol in mouse hepatic stellate cells. Alcohol Clin Exp Res. 2012 Nov; 36(11):1873-81.
14. Chen X, Guo Z, Okoro EU, Zhang H, Zhou L, Lin X, Rollins AT, Yang H. Up-regulation of ATP binding cassette transporter A1 expression by very low density lipoprotein receptor and apolipoprotein E receptor 2. J Biol Chem. 2012 Feb 03; 287(6):3751-9.
15. Lin X, Yang H, Zhou L, Guo Z. Nrf2-dependent induction of NQO1 in mouse aortic endothelial cells overexpressing catalase. Free Radic Biol Med. 2011 Jul 01; 51(1):97-106.
16. Chen X, Zhao Y, Guo Z, Zhou L, Okoro EU, Yang H. Transcriptional regulation of ATP-binding cassette transporter A1 expression by a novel signaling pathway. J Biol Chem. 2011 Mar 18; 286(11):8917-23.
17. Zhao Y, Guo Z, Lin X, Zhou L, Okoro EU, Fan G, Ramaswamy R, Yang H. Apolipoprotein E-Deficient Lipoproteins Induce Foam Cell Formation by Activation of PERK-EIF-2a Signaling Cascade. J Bioanal Biomed. 2010 Oct 06; 2:113-120.
18. Chen A, Guo Z, Zhou L, Yang H. Hepatic Endosome Protein Profiling in Apolipoprotein E Deficient Mice Expressing Apolipoprotein B48 but not B100. J Bioanal Biomed. 2010 Sep 23; 2(5):100-106.
19. Tang T, Lin X, Yang H, Zhou L, Wang Z, Shan G, Guo Z. Overexpression of antioxidant enzymes upregulates aryl hydrocarbon receptor expression via increased Sp1 DNA-binding activity. Free Radic Biol Med. 2010 Aug 01; 49(3):487-92.
20. Wang Z, Yang H, Ramesh A, Roberts LJ, Zhou L, Lin X, Zhao Y, Guo Z. Overexpression of Cu/Zn-superoxide dismutase and/or catalase accelerates benzo(a)pyrene detoxification by upregulation of the aryl hydrocarbon receptor in mouse endothelial cells. Free Radic Biol Med. 2009 Oct 15; 47(8):1221-9.
21. Yang H, Zhou L, Wang Z, Roberts LJ, Lin X, Zhao Y, Guo Z. Overexpression of antioxidant enzymes in ApoE-deficient mice suppresses benzo(a)pyrene-accelerated atherosclerosis. Atherosclerosis. 2009 Nov; 207(1):51-8.
22. Onumah OE, Jules GE, Zhao Y, Zhou L, Yang H, Guo Z. Overexpression of catalase delays G0/G1- to S-phase transition during cell cycle progression in mouse aortic endothelial cells. Free Radic Biol Med. 2009 Jun 15; 46(12):1658-67.
23. Mukherjee S, Stone WL, Yang H, Smith MG, Das SK. Protection of half sulfur mustard gas-induced lung injury in guinea pigs by antioxidant liposomes. J Biochem Mol Toxicol. 2009 Mar-Apr; 23(2):143-53.
24. Wu D, Yang H, Zhao Y, Sharan C, Goodwin JS, Zhou L, Guo Y, Guo Z. 2-Aminopurine inhibits lipid accumulation induced by apolipoprotein E-deficient lipoprotein in macrophages: potential role of eukaryotic initiation factor-2alpha phosphorylation in foam cell formation. J Pharmacol Exp Ther. 2008 Aug; 326(2):395-405.
25. Guo Z, Ran Q, Roberts LJ, Zhou L, Richardson A, Sharan C, Wu D, Yang H. Suppression of atherogenesis by overexpression of glutathione peroxidase-4 in apolipoprotein E-deficient mice. Free Radic Biol Med. 2008 Feb 01; 44(3):343-52.
26. Wu D, Sharan C, Yang H, Goodwin JS, Zhou L, Grabowski GA, Du H, Guo Z. Apolipoprotein E-deficient lipoproteins induce foam cell formation by downregulation of lysosomal hydrolases in macrophages. J Lipid Res. 2007 Dec; 48(12):2571-8.
27. Hou R, Chen F, Yang Y, Cheng X, Gao Z, Yang HO, Wu W, Mao T. Comparative study between coral-mesenchymal stem cells-rhBMP-2 composite and auto-bone-graft in rabbit critical-sized cranial defect model. J Biomed Mater Res A. 2007 Jan; 80(1):85-93.
28. Zhou L, Xiang W, Potts J, Floyd M, Sharan C, Yang H, Ross J, Nyanda AM, Guo Z. Reduction in extracellular superoxide dismutase activity in African-American patients with hypertension. Free Radic Biol Med. 2006 Nov 01; 41(9):1384-91.
29. Zhou L, Shi M, Guo Z, Brisbon W, Hoover R, Yang H. Different cytotoxic injuries induced by lysophosphatidylcholine and 7-ketocholesterol in mouse endothelial cells. Endothelium. 2006 May-Jun; 13(3):213-26.
30. Wu D, Yang H, Xiang W, Zhou L, Shi M, Julies G, Laplante JM, Ballard BR, Guo Z. Heterozygous mutation of ataxia-telangiectasia mutated gene aggravates hypercholesterolemia in apoE-deficient mice. J Lipid Res. 2005 Jul; 46(7):1380-7.
31. Yang H, Roberts LJ, Shi MJ, Zhou LC, Ballard BR, Richardson A, Guo ZM. Retardation of atherosclerosis by overexpression of catalase or both Cu/Zn-superoxide dismutase and catalase in mice lacking apolipoprotein E. Circ Res. 2004 Nov 26; 95(11):1075-81.
32. Van Remmen H, Qi W, Sabia M, Freeman G, Estlack L, Yang H, Mao Guo Z, Huang TT, Strong R, Lee S, Epstein CJ, Richardson A. Multiple deficiencies in antioxidant enzymes in mice result in a compound increase in sensitivity to oxidative stress. Free Radic Biol Med. 2004 Jun 15; 36(12):1625-34.
33. Shi M, Yang H, Motley ED, Guo Z. Overexpression of Cu/Zn-superoxide dismutase and/or catalase in mice inhibits aorta smooth muscle cell proliferation. Am J Hypertens. 2004 May; 17(5 Pt 1):450-6.
34. Yang H, Shi M, Story J, Richardson A, Guo Z. Food restriction attenuates age-related increase in the sensitivity of endothelial cells to oxidized lipids. J Gerontol A Biol Sci Med Sci. 2004 Apr; 59(4):316-23.
35. Yang H, Shi M, Richardson A, Vijg J, Guo Z. Attenuation of leukocyte-endothelium interaction by antioxidant enzymes. Free Radic Biol Med. 2003 Aug 01; 35(3):266-76.
36. Uddin M, Yang H, Shi M, Polley-Mandal M, Guo Z. Elevation of oxidative stress in the aorta of genetically hypertensive mice. Mech Ageing Dev. 2003 Jul; 124(7):811-7.
37. Yang H, Shi M, VanRemmen H, Chen X, Vijg J, Richardson A, Guo Z. Reduction of pressor response to vasoconstrictor agents by overexpression of catalase in mice. Am J Hypertens. 2003 Jan; 16(1):1-5.
38. Guo Z, Mitchell-Raymundo F, Yang H, Ikeno Y, Nelson J, Diaz V, Richardson A, Reddick R. Dietary restriction reduces atherosclerosis and oxidative stress in the aorta of apolipoprotein E-deficient mice. Mech Ageing Dev. 2002 Apr 30; 123(8):1121-31.
39. Guo ZM, Yang H, Hamilton ML, VanRemmen H, Richardson A. Effects of age and food restriction on oxidative DNA damage and antioxidant enzyme activities in the mouse aorta. Mech Ageing Dev. 2001 Oct; 122(15):1771-86.
40. Van Remmen H, Williams MD, Guo Z, Estlack L, Yang H, Carlson EJ, Epstein CJ, Huang TT, Richardson A. Knockout mice heterozygous for Sod2 show alterations in cardiac mitochondrial function and apoptosis. Am J Physiol Heart Circ Physiol. 2001 Sep; 281(3):H1422-32.
41. Hamilton ML, Van Remmen H, Drake JA, Yang H, Guo ZM, Kewitt K, Walter CA, Richardson A. Does oxidative damage to DNA increase with age? Proc Natl Acad Sci U S A. 2001 Aug 28; 98(18):10469-74.
42. Guo Z, Van Remmen H, Yang H, Chen X, Mele J, Vijg J, Epstein CJ, Ho YS, Richardson A. Changes in expression of antioxidant enzymes affect cell-mediated LDL oxidation and oxidized LDL-induced apoptosis in mouse aortic cells. Arterioscler Thromb Vasc Biol. 2001 Jul; 21(7):1131-8.
43. Van Remmen H, Salvador C, Yang H, Huang TT, Epstein CJ, Richardson A. Characterization of the antioxidant status of the heterozygous manganese superoxide dismutase knockout mouse. Arch Biochem Biophys. 1999 Mar 01; 363(1):91-7.
44. Guo Z, Heydari AR, Wu W, Yang H, Sabia MR, Richardson A. Characterization of gene-specific DNA repair by primary cultures of rat hepatocytes. J Cell Physiol. 1998 Aug; 176(2):314-22.
45. Van Remmen H, Williams MD, Yang H, Walter CA, Richardson A. Analysis of the transcriptional activity of the 5'-flanking region of the rat catalase gene in transiently transfected cells and in transgenic mice. J Cell Physiol. 1998 Jan; 174(1):18-26.
46. Guo ZM, Hang DH, Yang H. [Measurement for instantaneous acceleration of ventricular pressure and its significant]. Sheng Li Xue Bao. 1989 Feb; 41(1):102-10.

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1. Yang H, Guo ZM, Zhang SH . An application of the first and second derivative impedance cardiogram for evaluating myocardial ischemia. J. Chinese Physics in Medicine. 1986; (7):242-248.
2. Yang H, Zhang SH . Analysis of the first and second derivative impedance cardiogram of human. Wuhan Medical Journal. 1984; (8):260-264.

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