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Shireen Saleque
Title Assistant Professor
Faculty Rank Assistant Professor
Degree Ph.D
Institution City College, CUNY
Department Biology
Clusters Cancer
Infectious and Immunological Diseases
MR 716
160 Convent Avenue
City New York
State NY
Postal Code 10031
Telephone 212-650-8538
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  Our laboratory focusses on the elucidation of the molecular mechanisms of hematopoietic development during various stages of organismal (embryonic versus adult) and cellular (stem, progenitor, and effector) development in mammals. This includes determination of molecular players and events, both genetic and epigenetic, regulating commitment, generation, differentiation and function of multiple blood lineage cells. These investigations involve the murine model system and also in vitro manipulation of primary and cultured (transformed) cells.
Recent work in the lab has illustrated novel regulatory mechanisms for fine tuning epigenetic activity and modulating cellular differentiation during normal erythroid (red blood cell) and megakaryocytic (platelet lineage) development. These processes are crucial for optimal gene expression during normal development and could be potentially applicable for therapeutic intervention in blood cell disorders and malignancies. Recently, the laboratory research has also diversified to explore intersections between transcriptional regulation, chromatin function, signal transduction cascades and cytoskeletal alterations in co-ordinating lineage specification and determination. These processes will be examined in detail upon in vivo genetic ablation or manipulation of candidate genes. In parallel new players and pathways involved in the above developmental systems and processes will be identified by chromatin immmunoprecipitation (ChIPseq), expression profiling (RNAseq) and protein-protein interaction (affinity purification and mass spectrometric analysis) screens and the newly identified candidates characterized for their biochemical activities and physiological functions.

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  Research projects funded by the National Institutes of Health (NIH), the Centers for Disease Control (CDC), the Food and Drug Administration (FDA), and the Department of Veterans Affairs (VA)

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2019Molecular Regulation of Mammalian Erythro-Megakaryocytic Development5SC1HL136277-03
2018Molecular Regulation of Mammalian Erythro-Megakaryocytic Development5SC1HL136277-02
2017Molecular Regulation of Mammalian Erythro-Megakaryocytic Development1SC1HL136277-01
2014Genetic and Epigenetic Regulation of Hematopoiesis.5SC1HL104638-05
2013Genetic and Epigenetic Regulation of Hematopoiesis.5SC1HL104638-04
2012Genetic and Epigenetic Regulation of Hematopoiesis.5SC1HL104638-03
2011Genetic and Epigenetic Regulation of Hematopoiesis.5SC1HL104638-02
2010Genetic and Epigenetic Regulation of Hematopoiesis.1SC1HL104638-01

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1. Singh D, Upadhyay G, Sengupta A, Biplob MA, Chakyayil S, George T, Saleque S. Cooperative Stimulation of Megakaryocytic Differentiation by Gfi1b Gene Targets Kindlin3 and Talin1. PLoS One. 2016; 11(10):e0164506.
2. Sengupta A, Upadhyay G, Sen S, Saleque S. Reciprocal regulation of alternative lineages by Rgs18 and its transcriptional repressor Gfi1b. J Cell Sci. 2016 Jan 01; 129(1):145-54.
3. Upadhyay G, Chowdhury AH, Vaidyanathan B, Kim D, Saleque S. Antagonistic actions of Rcor proteins regulate LSD1 activity and cellular differentiation. Proc Natl Acad Sci U S A. 2014 Jun 03; 111(22):8071-6.
4. Small C, Ramroop J, Otazo M, Huang LH, Saleque S, Govind S. An unexpected link between notch signaling and ROS in restricting the differentiation of hematopoietic progenitors in Drosophila. Genetics. 2014 Jun; 197(2):471-83.
5. Chowdhury AH, Ramroop JR, Upadhyay G, Sengupta A, Andrzejczyk A, Saleque S. Differential transcriptional regulation of meis1 by Gfi1b and its co-factors LSD1 and CoREST. PLoS One. 2013; 8(1):e53666.
6. Saleque S, Kim J, Rooke HM, Orkin SH. Epigenetic regulation of hematopoietic differentiation by Gfi-1 and Gfi-1b is mediated by the cofactors CoREST and LSD1. Mol Cell. 2007 Aug 17; 27(4):562-72.
7. Zhou J, Saleque S, Ermakova O, Sepulveda MA, Yang Q, Eckhardt LA, Schildkraut CL, Birshtein BK. Changes in replication, nuclear location, and expression of the Igh locus after fusion of a pre-B cell line with a T cell line. J Immunol. 2005 Aug 15; 175(4):2317-20.
8. Hock H, Hamblen MJ, Rooke HM, Schindler JW, Saleque S, Fujiwara Y, Orkin SH. Gfi-1 restricts proliferation and preserves functional integrity of haematopoietic stem cells. Nature. 2004 Oct 21; 431(7011):1002-7.
9. Gordon SJ, Saleque S, Birshtein BK. Yin Yang 1 is a lipopolysaccharide-inducible activator of the murine 3' Igh enhancer, hs3. J Immunol. 2003 Jun 01; 170(11):5549-57.
10. Saleque S, Cameron S, Orkin SH. The zinc-finger proto-oncogene Gfi-1b is essential for development of the erythroid and megakaryocytic lineages. Genes Dev. 2002 Feb 01; 16(3):301-6.
11. Saleque S, Singh M, Birshtein BK. Ig heavy chain expression and class switching in vitro from an allele lacking the 3' enhancers DNase I-hypersensitive hs3A and hs1,2. J Immunol. 1999 Mar 01; 162(5):2791-803.
12. Saleque S, Singh M, Little RD, Giannini SL, Michaelson JS, Birshtein BK. Dyad symmetry within the mouse 3' IgH regulatory region includes two virtually identical enhancers (C alpha3'E and hs3). J Immunol. 1997 May 15; 158(10):4780-7.
13. Birshtein BK, Chen C, Saleque S, Michaelson JS, Singh M, Little RD. Murine and human 3'IgH regulatory sequences. Curr Top Microbiol Immunol. 1997; 224:73-80.
14. Shrivastava A, Saleque S, Kalpana GV, Artandi S, Goff SP, Calame K. Inhibition of transcriptional regulator Yin-Yang-1 by association with c-Myc. Science. 1993 Dec 17; 262(5141):1889-92.
15. Riggs KJ, Saleque S, Wong KK, Merrell KT, Lee JS, Shi Y, Calame K. Yin-yang 1 activates the c-myc promoter. Mol Cell Biol. 1993 Dec; 13(12):7487-95.
16. Saleque S, Ruiz N, Drickamer K. Expression and characterization of a carbohydrate-binding fragment of rat aggrecan. Glycobiology. 1993 Apr; 3(2):185-90.

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