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Jarrett, Harry
One or more keywords matched the following properties of Jarrett, Harry
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overview Signal transduction, and more specifically the role of Ca2+ and its receptor protein, calmodulin, in cellular regulation We have shown that dystrophin, the protein product of the Duchenne muscular dystrophy gene locus, is a specific calmodulin-binding protein and discovered a signal transduction pathway, which links laminin-a-dystroglycan-ß-dystroglycan-dystrophin-syntrophin-Grb2-Sos1-Rac1-PAK1-MKK4/7-JNK, causing c-jun phosphorylation and activation. This work is supported by National Institutes of Health grant 1R01AR051440. Transcription factor purification and characterization We first coupled DNA to HPLC silica and have made a significant contribution to the purification and characterization of transcription factors. Most recently we developed systematic oligonucleotide trapping, a method for purifying transcription factors to near homogeneity in a single step. Current experiments involve promoter trapping, a method which interfaces DNA-HPLC with proteomic techniques (MALDI-TOF and ESE-ion trap) to characterize the transcription complex of the c-jun promoter. This work is supported by National Institutes of Health grant 1R01GM43609.
One or more keywords matched the following items that are connected to Jarrett, Harry
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Academic Article Methods for proteomic analysis of transcription factors.
Academic Article Purification and identification of a transcription factor, USF-2, binding to E-box element in the promoter of human telomerase reverse transcriptase (hTERT).
Academic Article Transcription factor proteomics: identification by a novel gel mobility shift-three-dimensional electrophoresis method coupled with southwestern blot and high-performance liquid chromatography-electrospray-mass spectrometry analysis.
Academic Article Repeated probing of Southwestern blots using alkaline phosphatase stripping.
Academic Article Temperature dependence of DNA affinity chromatography of transcription factors.
Academic Article Proteomic methodologies to study transcription factor function.
Academic Article CCR5 promoter haplotype transcription complex characterization.
Academic Article Laminin-a1 LG4-5 domain binding to dystroglycan mediates muscle cell survival, growth, and the AP-1 and NF-?B transcription factors but also has adverse effects.
Academic Article Purification and characterization of transcription factors.
Academic Article A novel SP1/SP3 dependent intronic enhancer governing transcription of the UCP3 gene in brown adipocytes.
Academic Article Coupling of deoxyribonucleic acid to solid supports using 3' terminal ribose incorporation.
Academic Article Promoter trapping method: transcription factor purification using human telomerase reverse transcriptase promoter.
Academic Article Method for trapping affinity chromatography of transcription factors using aldehyde-hydrazide coupling to agarose.
Academic Article Southwestern Blotting Assay.
Academic Article Transcription factor-green fluorescent protein chimeric fusion proteins and their use in studies of DNA affinity chromatography.
Academic Article Heparin elution of transcription factors from DNA-Sepharose columns.
Academic Article DNA affinity chromatography of transcription factors.
Academic Article Methods for transcription factor separation.
Academic Article Oligonucleotide trapping method for purification of transcription factors.
Academic Article Members of the large Maf transcription family regulate insulin gene transcription in islet beta cells.
Academic Article Effect of the detergent Tween-20 on the DNA affinity chromatography of Gal4, C/EBPalpha, and lac repressor with observations on column regeneration.
Academic Article Oligonucleotide trapping method for transcription factor purification systematic optimization using electrophoretic mobility shift assay.
Academic Article Promoter trapping of c-jun promoter-binding transcription factors.
Academic Article Novel DNA-Sepharose purification of the FadR transcription factor.
Academic Article Trapping of transcription factors with symmetrical DNA using thiol-disulfide exchange chemistry.
Academic Article Two-dimensional southwestern blotting and characterization of transcription factors on-blot.
Concept Transcription Factors
Concept Sp1 Transcription Factor
Concept Transcription Factor AP-1
Concept p300-CBP Transcription Factors
Concept MafB Transcription Factor
Concept Maf Transcription Factors, Large
Concept Sp3 Transcription Factor
Search Criteria
  • Transcription Factors
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