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Ana M. Espino

TitlePhD
Faculty RankAssociate Professor
InstitutionUniversity of Puerto Rico
DepartmentSchool of Medicine
AddressUPR-Medical Sciences Campus, School of Medicine
San Juan PR 936
Phone7877582525 ext. 1309
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    Dr. Ana Espino is an associate professor in the Department of Microbiology, Laboratory of Molecular Parasitology and Immunology at the School of Medicine, University of Puerto Rico Medical Sciences Campus.

    Dr. Espino earned her Ph.D. degree in Parasitology and Biochemestry from the Pedro Kouri Institute of Tropical Medicine in Havana, Cuba in 1997, where she also completed post-doctoral training in Molecular Biology and Immunology. She also has a post doctoral in Molecular Parasitology from the University of Puerto Rico Medical Sciences Campus.

    Dr. Espino’s field of interest is molecular and parasite immunology, Fascioliasis, caused by Fasciola hepatica, represents a recognized unsolved agricultural problem responsible for economic losses estimated in around US $3 billion per year to rural agricultural communities and commercial producers worldwide including the United States. Recent reports indicate that F. hepatica is also a major human pathogen and is increasingly recognized as a cause of significant human diseases, with 17 million people infected. Schistosomiasis, the second major parasitic disease in the world after malaria, is a chronic, debilitating and sometimes fatal disease affecting 200 and 300 million persons worldwide. Vaccines represent the most attractive long-term alternative to control fascioliasis and
    schistosomiasis.

    Three projects are ongoing in Dr. Espino’s laboratory. They relate to the identification, purification and molecular characterization of Fasciola hepatica and Schistosoma mansoni antigens with immunoprophylactic potential; the role of the Toll-like receptors (TLRs) in the interaction with excretory secretory antigens of F. hepatica at early stage of infection, and the elucidation of the immune mechanism involved in the protection induced by two novel DNA vaccines against F. hepatica, as well as the assessment of the protection level conferred by these proteins in experimental models. Dr. Espino has filled two patent applications to the United States Patents and Trademarks Office. Dr. Espino’s research has been funded by the MBRS-SCORE Program from 2000-2004, an NIH-NIAID R21 grant from 2003-2006, and an RCMI Pilot Project from 2006-2009.

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    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Espino A, Gouilly J, Chen Q, Colin P, Guerby P, Izopet J, Amara A, Tabiasco J, Al-Daccak R, El Costa H, Jabrane-Ferrat N. The mechanisms underlying the immune control of Zika virus infection at the maternal-fetal interface. Front Immunol. 2022; 13:1000861. PMID: 36483552.
      Citations: 1     Fields:    
    2. Karger AB, Brien JD, Christen JM, Dhakal S, Kemp TJ, Klein SL, Pinto LA, Premkumar L, Roback JD, Binder RA, Boehme KW, Boppana S, Cordon-Cardo C, Crawford JM, Daiss JL, Dupuis AP, Espino AM, Firpo-Betancourt A, Forconi C, Forrest JC, Girardin RC, Granger DA, Granger SW, Haddad NS, Heaney CD, Hunt DT, Kennedy JL, King CL, Krammer F, Kruczynski K, LaBaer J, Lee FE, Lee WT, Liu SL, Lozanski G, Lucas T, Mendu DR, Moormann AM, Murugan V, Okoye NC, Pantoja P, Payne AF, Park J, Pinninti S, Pinto AK, Pisanic N, Qiu J, Sariol CA, Simon V, Song L, Steffen TL, Stone ET, Styer LM, Suthar MS, Thomas SN, Thyagarajan B, Wajnberg A, Yates JL, Sobhani K. The Serological Sciences Network (SeroNet) for COVID-19: Depth and Breadth of Serology Assays and Plans for Assay Harmonization. mSphere. 2022 08 31; 7(4):e0019322. PMID: 35703544.
      Citations: 7     Fields:    Translation:HumansCells
    3. Figueiredo JC, Hirsch FR, Kushi LH, Nembhard WN, Crawford JM, Mantis N, Finster L, Merin NM, Merchant A, Reckamp KL, Melmed GY, Braun J, McGovern D, Parekh S, Corley DA, Zohoori N, Amick BC, Du R, Gregersen PK, Diamond B, Taioli E, Sariol C, Espino A, Weiskopf D, Gifoni A, Brien J, Hanege W, Lipsitch M, Zidar DA, Scheck McAlearney A, Wajnberg A, LaBaer J, Yvonne Lewis E, Binder RA, Moormann AM, Forconi C, Forrester S, Batista J, Schieffelin J, Kim D, Biancon G, VanOudenhove J, Halene S, Fan R, Barouch DH, Alter G, Pinninti S, Boppana SB, Pati SK, Latting M, Karaba AH, Roback J, Sekaly R, Neish A, Brincks AM, Granger DA, Karger AB, Thyagarajan B, Thomas SN, Klein SL, Cox AL, Lucas T, Furr-Holden D, Key K, Jones N, Wrammerr J, Suthar M, Yu Wong S, Bowman NM, Simon V, Richardson LD, McBride R, Krammer F, Rana M, Kennedy J, Boehme K, Forrest C, Granger SW, Heaney CD, Knight Lapinski M, Wallet S, Baric RS, Schifanella L, Lopez M, Fern?ndez S, Kenah E, Panchal AR, Britt WJ, Sanz I, Dhodapkar M, Ahmed R, Bartelt LA, Markmann AJ, Lin JT, Hagan RS, Wolfgang MC, Skarbinski J. Mission, Organization, and Future Direction of the Serological Sciences Network for COVID-19 (SeroNet) Epidemiologic Cohort Studies. Open Forum Infect Dis. 2022 Jun; 9(6):ofac171. PMID: 35765315.
      Citations:    
    4. Karger AB, Brien JD, Christen JM, Dhakal S, Kemp TJ, Klein SL, Pinto LA, Premkumar L, Roback JD, Binder RA, Boehme KW, Boppana S, Cordon-Cardo C, Crawford JM, Daiss JL, Dupuis AP, Espino AM, Firpo-Betancourt A, Forconi C, Forrest JC, Girardin RC, Granger DA, Granger SW, Haddad NS, Heaney CD, Hunt DT, Kennedy JL, King CL, Krammer F, Kruczynski K, LaBaer J, Lee FE, Lee WT, Liu SL, Lozanski G, Lucas T, Mendu DR, Moormann AM, Murugan V, Okoye NC, Pantoja P, Payne AF, Park J, Pinninti S, Pinto AK, Pisanic N, Qiu J, Sariol CA, Simon V, Song L, Steffen TL, Stone ET, Styer LM, Suthar MS, Thomas SN, Thyagarajan B, Wajnberg A, Yates JL, Sobhani K. The Serological Sciences Network (SeroNet) for COVID-19: Depth and Breadth of Serology Assays and Plans for Assay Harmonization. medRxiv. 2022 Mar 14. PMID: 35262095.
      Citations:    
    5. Sariol CA, Serrano-Collazo C, Ortiz EJ, Pantoja P, Cruz L, Arana T, Atehortua D, Pabon-Carrero C, Espino AM. Limited Impact of Delta Variant's Mutations on the Effectiveness of Neutralization Conferred by Natural Infection or COVID-19 Vaccines in a Latino Population. Viruses. 2021 11 30; 13(12). PMID: 34960674.
      Citations: 2     Fields:    Translation:HumansCellsPHPublic Health
    6. Ruiz-Jim?nez C, Celias D, Vald?s B, Ramos-P?rez WD, Cervi L, Espino AM. Fasciola hepatica fatty acid binding protein (Fh12) induces apoptosis and tolerogenic properties in murine bone marrow derived dendritic cells. Exp Parasitol. 2021 Dec; 231:108174. PMID: 34752732.
      Citations: 1     Fields:    Translation:AnimalsCells
    7. Sariol CA, Serrano-Collazo C, Ortiz EJ, Pantoja P, Cruz L, Arana T, Atehortua D, Pabon-Carrero C, Espino AM. Limited impact of Delta variant’s mutations in the effectiveness of neutralization conferred by natural infection or COVID-19 vaccines in a Latino population. medRxiv. 2021 Oct 26. PMID: 34729566.
      Citations:    
    8. Sariol CAA, Pantoja P, Serrano-Collazo C, Rosa-Arocho T, Armina-Rodr?guez A, Cruz L, Stone ETT, Arana T, Climent C, Latoni G, Atehortua D, Pabon-Carrero C, Pinto AKK, Brien JDD, Espino AMM. Function Is More Reliable than Quantity to Follow Up the Humoral Response to the Receptor-Binding Domain of SARS-CoV-2-Spike Protein after Natural Infection or COVID-19 Vaccination. Viruses. 2021 09 30; 13(10). PMID: 34696403.
      Citations: 15     Fields:    Translation:HumansCellsPHPublic Health
    9. Rodr?guez-Martin? E, Medina-Prieto R, Santana-Bagur J, Sant? M, Pantoja P, Espino AM, Sariol CA, Torres EA. Early immunologic response to mRNA COVID-19 vaccine in patients receiving biologics and/or immunomodulators. medRxiv. 2021 Sep 15. PMID: 34545370.
      Citations:    
    10. Sariol CA, Pantoja P, Serrano-Collazo C, Rosa-Arocho T, Armina A, Cruz L, Stone ET, Arana T, Climent C, Latoni G, Atehortua D, Pabon-Carrero C, Pinto AK, Brien JD, Espino AM. Function is more reliable than quantity to follow up the humoral response to the Receptor Binding Domain of SARS- CoV-2 Spike protein after natural infection or COVID-19 vaccination. medRxiv. 2021 Aug 10. PMID: 34100029.
      Citations:    
    11. Vald?s-Fern?ndez BN, Duconge J, Espino AM, Rua?o G. Personalized health and the coronavirus vaccines-Do?individual genetics matter? Bioessays. 2021 09; 43(9):e2100087. PMID: 34309055.
      Citations: 5     Fields:    Translation:HumansCellsPHPublic Health
    12. Espino A, El Costa H, Tabiasco J, Al-Daccak R, Jabrane-Ferrat N. Innate Immune Response to Viral Infections at the Maternal-Fetal Interface in Human Pregnancy. Front Med (Lausanne). 2021; 8:674645. PMID: 34368184.
      Citations:    
    13. Chen Q, Gouilly J, Ferrat YJ, Espino A, Glaziou Q, Cartron G, El Costa H, Al-Daccak R, Jabrane-Ferrat N. Metabolic reprogramming by Zika virus provokes inflammation in human placenta. Nat Commun. 2020 06 11; 11(1):2967. PMID: 32528049.
      Citations: 31     Fields:    Translation:HumansCellsPHPublic Health
    14. Figueroa-Santiago O, Espino A. Purification of Native Fasciola hepatica Fatty Acid-Binding Protein and Induction of Alternative Activation of Human Macrophages. Methods Mol Biol. 2020; 2137:149-169. PMID: 32399927.
      Citations:    Fields:    Translation:HumansAnimalsCells
    15. Figueroa-Santiago O, Espino A. Possible Role for Toll-Like Receptors in Interaction of Fasciola hepatica Excretory-Secretory Products with Human Monocyte Cell Line. Methods Mol Biol. 2020; 2137:171-180. PMID: 32399928.
      Citations:    Fields:    Translation:HumansAnimalsCells
    16. Rosado-Franco JJ, Ramos-Benitez MJ, Parodi LM, Rosario D, Compo N, Giavedoni LD, Espino AM. Outlining key inflammation-associated parameters during early phase of an experimental gram-negative sepsis model in rhesus macaques (Macaca mulatta). Animal Model Exp Med. 2019 Dec; 2(4):326-333. PMID: 31942564.
      Citations: 1     Fields:    
    17. Aguayo V, Vald?s Fernandez BN, Rodr?guez-Valent?n M, Ruiz-Jim?nez C, Ramos-Ben?tez MJ, M?ndez LB, Espino AM. Fasciola hepatica GST downregulates NF-?B pathway effectors and inflammatory cytokines while promoting survival in a mouse septic shock model. Sci Rep. 2019 02 19; 9(1):2275. PMID: 30783117.
      Citations: 11     Fields:    Translation:HumansAnimalsCells
    18. Ramos-Benitez MJ, Ruiz-Jimenez C, Rosado-Franco JJ, Ramos-P?rez WD, Mendez LB, Osuna A, Espino AM. Fh15 Blocks the Lipopolysaccharide-Induced Cytokine Storm While Modulating Peritoneal Macrophage Migration and CD38 Expression within Spleen Macrophages in a Mouse Model of Septic Shock. mSphere. 2018 12 19; 3(6). PMID: 30567900.
      Citations: 8     Fields:    Translation:AnimalsCells
    19. Ramos-Ben?tez MJ, Lopez-Cruz LM, Aguayo V, Ruiz-Jimenez C, Espino AM. Cell-free expression, purification and immunoreactivity assessment of recombinant Fasciola hepatica saposin-like protein-2. Mol Biol Rep. 2018 Oct; 45(5):1551-1556. PMID: 30032380.
      Citations: 2     Fields:    Translation:HumansAnimalsCells
    20. Aguayo V, Valdes B, Espino AM. Assessment of Fasciola hepatica glutathione S-transferase as an antigen for serodiagnosis of human chronic fascioliasis. Acta Trop. 2018 Oct; 186:41-49. PMID: 29990477.
      Citations: 6     Fields:    Translation:HumansAnimals
    21. Ramos-Ben?tez MJ, Ruiz-Jim?nez C, Aguayo V, Espino AM. Recombinant Fasciola hepatica fatty acid binding protein suppresses toll-like receptor stimulation in response to multiple bacterial ligands. Sci Rep. 2017 07 14; 7(1):5455. PMID: 28710478.
      Citations: 13     Fields:    Translation:HumansAnimalsCells
    22. Figueroa-Santiago O, Espino AM. Fasciola hepatica ESPs Could Indistinctly Activate or Block Multiple Toll-Like Receptors in a Human Monocyte Cell Line. Ann Clin Pathol. 2017; 5(3). PMID: 29152559.
      Citations:    
    23. Shin SH, Hsu A, Chastain HM, Cruz LA, Elder ES, Sapp SG, McAuliffe I, Espino AM, Handali S. Development of Two FhSAP2 Recombinant-Based Assays for Immunodiagnosis of Human Chronic Fascioliasis. Am J Trop Med Hyg. 2016 Oct 05; 95(4):852-855. PMID: 27549636.
      Citations: 10     Fields:    Translation:HumansAnimals
    24. De Pablos LM, D?az Lozano IM, Jercic MI, Quinzada M, Gim?nez MJ, Calabuig E, Espino AM, Schijman AG, Zulantay I, Apt W, Osuna A. The C-terminal region of Trypanosoma cruzi MASPs is antigenic and secreted via exovesicles. Sci Rep. 2016 06 08; 6:27293. PMID: 27270330.
      Citations: 20     Fields:    Translation:HumansAnimalsCells
    25. Rivera F, Espino AM. Adjuvant-enhanced antibody and cellular responses to inclusion bodies expressing FhSAP2 correlates with protection of mice to Fasciola hepatica. Exp Parasitol. 2016 Jan; 160:31-8. PMID: 26632503.
      Citations: 7     Fields:    Translation:AnimalsCellsPHPublic Health
    26. Martin I, Cab?n-Hern?ndez K, Figueroa-Santiago O, Espino AM. Fasciola hepatica fatty acid binding protein inhibits TLR4 activation and suppresses the inflammatory cytokines induced by lipopolysaccharide in vitro and in vivo. J Immunol. 2015 Apr 15; 194(8):3924-36. PMID: 25780044.
      Citations: 48     Fields:    Translation:AnimalsCells
    27. Figueroa-Santiago O, Espino AM. Fasciola hepatica fatty acid binding protein induces the alternative activation of human macrophages. Infect Immun. 2014 Dec; 82(12):5005-12. PMID: 25225247.
      Citations: 28     Fields:    Translation:HumansAnimalsCells
    28. Cab?n-Hern?ndez K, Gaudier JF, Ruiz-Jim?nez C, Espino AM. Development of two antibody detection enzyme-linked immunosorbent assays for serodiagnosis of human chronic fascioliasis. J Clin Microbiol. 2014 Mar; 52(3):766-72. PMID: 24353000.
      Citations: 8     Fields:    Translation:HumansAnimals
    29. Cab?n-Hern?ndez K, Espino AM. Differential expression and localization of saposin-like protein 2 of Fasciola hepatica. Acta Trop. 2013 Dec; 128(3):591-7. PMID: 23988299.
      Citations: 6     Fields:    Translation:HumansAnimalsCells
    30. Morales A, Espino AM. Evaluation and characterization of Fasciola hepatica tegument protein extract for serodiagnosis of human fascioliasis. Clin Vaccine Immunol. 2012 Nov; 19(11):1870-8. PMID: 23015645.
      Citations: 12     Fields:    Translation:HumansAnimals
    31. Gaudier JF, Cab?n-Hern?ndez K, Osuna A, Espino AM. Biochemical characterization and differential expression of a 16.5-kilodalton tegument-associated antigen from the liver fluke Fasciola hepatica. Clin Vaccine Immunol. 2012 Mar; 19(3):325-33. PMID: 22278327.
      Citations: 11     Fields:    Translation:HumansAnimalsCells
    32. Cab?n-Hern?ndez K, Gaudier JF, Espino AM. Characterization and differential expression of a ferritin protein from Fasciola hepatica. Mol Biochem Parasitol. 2012 Mar-Apr; 182(1-2):54-61. PMID: 22240114.
      Citations: 8     Fields:    Translation:AnimalsCells
    33. Figueroa-Santiago O, Delgado B, Espino AM. Fasciola hepatica saposin-like protein-2-based ELISA for the serodiagnosis of chronic human fascioliasis. Diagn Microbiol Infect Dis. 2011 Jul; 70(3):355-61. PMID: 21683266.
      Citations: 20     Fields:    Translation:HumansAnimals
    34. Miralles F, Tarong? S, Espino A. Quantitative evaluation of the drawing of a spiral on a paper. Mov Disord. 2011 Jun; 26(7):1369; author reply 1369-70. PMID: 21506161.
      Citations: 1     Fields:    Translation:Humans
    35. Boukli NM, Delgado B, Ricaurte M, Espino AM. Fasciola hepatica and Schistosoma mansoni: identification of common proteins by comparative proteomic analysis. J Parasitol. 2011 Oct; 97(5):852-61. PMID: 21506812.
      Citations: 11     Fields:    Translation:HumansAnimals
    36. Espino AM, Morales A, Delgado B, Rivera FM, Figueroa O, Su?rez E. Partial immunity to Fasciola hepatica in mice after vaccination with FhSAP2 delivered as recombinant protein or DNA construct. Ethn Dis. 2010; 20(1 Suppl 1):S1-17-23. PMID: 20521379.
      Citations: 11     Fields:    Translation:AnimalsPHPublic Health
    37. Espino AM, Rivera F. Quantitation of cytokine mRNA by real-time RT-PCR during a vaccination trial in a rabbit model of fascioliasis. Vet Parasitol. 2010 Apr 19; 169(1-2):82-92. PMID: 20056331.
      Citations: 15     Fields:    Translation:AnimalsPHPublic Health
    38. Espino AM, Torres D, Morales A, Delgado B, Quetel J, Osuna A. Fasciola hepatica: identification of CD4+ T-helper epitopes from the 11.5 kDa saposin-like protein SAP-2 using synthetic peptides. Exp Parasitol. 2007 Sep; 117(1):65-73. PMID: 17475253.
      Citations: 3     Fields:    Translation:AnimalsCells
    39. Torres D, Espino AM. Mapping of B-cell epitopes on a novel 11.5-kilodalton Fasciola hepatica-Schistosoma mansoni cross-reactive antigen belonging to a member of the F. hepatica saposin-like protein family. Infect Immun. 2006 Aug; 74(8):4932-8. PMID: 16861685.
      Citations: 9     Fields:    Translation:AnimalsCells
    40. Espino AM, Osuna A, Gil R, Hillyer GV. Fasciola hepatica: humoral and cytokine responses to a member of the saposin-like protein family following delivery as a DNA vaccine in mice. Exp Parasitol. 2005 Aug; 110(4):374-83. PMID: 15907838.
      Citations: 13     Fields:    Translation:Animals
    41. Espino AM, Hillyer GV. A novel Fasciola hepatica saposinlike recombinant protein with immunoprophylactic potential. J Parasitol. 2004 Aug; 90(4):876-9. PMID: 15357089.
      Citations: 18     Fields:    Translation:Animals
    42. Espino AM, Hillyer GV. Molecular cloning of a member of the Fasciola hepatica saposin-like protein family. J Parasitol. 2003 Jun; 89(3):545-52. PMID: 12880256.
      Citations: 27     Fields:    Translation:HumansAnimalsCells
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