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Sudha Sharma

TitleAssociate Professor
Faculty RankAssociate Professor
InstitutionHoward University
DepartmentBiochemistry and Molecular Biology
Address520 W Street NW
Suite 3424D, Adams Bldg
College of Medicine
Washington DC 20059
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    My lab is interested in understanding how mammalian cells maintain genomic stability under normal growth conditions and following DNA damage, and how loss of genetic integrity contributes to cancer, premature aging and other diseases. We utilize a combination of biochemical, molecular and cell biological tools to examine functions of RecQ helicases, a group of highly conserved DNA unwinding enzymes described as caretakers of the genome and implicated in rare genetic diseases. For the past few years, my lab has been investigating investigating RECQ1 (also known as RECQL or RECQL1), the most abundant human RecQ homolog, with a goal to determine common and specialized functions of human RecQ helicases in mechanisms of genome maintenance. The overall focus is on elucidating how impaired function of a specific RecQ protein relates to disease outcomes, including cancer predisposition and premature aging. Following my earlier discovery that the loss of RECQ1 is sufficient to cause genomic instability in mammalian cells, recent work in my lab is investigating in detail the specific roles of RECQ1 in genome maintenance mechanisms of DNA repair and transcriptional regulation, and exploring its broader significance in cellular homeostasis. RECQ1 is frequently upregulated in various cancers and is especially important for cancer cell proliferation. Additionally, germline mutations in RECQ1 significantly increase susceptibility to breast cancer. Thus, we are also investigating RECQ1 as a modifier of cancer development, progression and chemotherapeutic response.

    Collapse Research 
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    SC1GM093999     (SHARMA, SUDHA)Sep 1, 2010 - Aug 31, 2021
    Molecular Analysis of RECQ1 Functions in Genome Maintenance
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Lu X, Redon CE, Tang W, Parvathaneni S, Bokhari B, Debnath S, Li XL, Muys BR, Song Y, Pongor LS, Sheikh O, Green AR, Madhusudan S, Lal A, Ambs S, Khan J, Aladjem MI, Sharma S. Genome-Wide Analysis Unveils DNA Helicase RECQ1 as a Regulator of Estrogen Response Pathway in Breast Cancer Cells. Mol Cell Biol. 2021 Jan 19. PMID: 33468559.
      Citations:    Fields:    
    2. Debnath S, Sharma S. RECQ1 Helicase in Genomic Stability and Cancer. Genes (Basel). 2020 06 05; 11(6). PMID: 32517021.
      Citations: 1     Fields:    
    3. Sharma S, Gupta P, Aggarwal R, Malhotra P, Minz RW, Bansal F. Demystifying Biclonal Gammopathy: A Pathologist's Perspective. Lab Med. 2019 Oct 10; 50(4):357-363. PMID: 30915470.
      Citations: 1     Fields:    Translation:Humans
    4. Sharma P, Alsharif S, Bursch K, Parvathaneni S, Anastasakis DG, Chahine J, Fallatah A, Nicolas K, Sharma S, Hafner M, Kallakury B, Chung BM. Keratin 19 regulates cell cycle pathway and sensitivity of breast cancer cells to CDK inhibitors. Sci Rep. 2019 10 10; 9(1):14650. PMID: 31601969.
      Citations: 2     Fields:    Translation:HumansCells
    5. Parvathaneni S, Sharma S. The DNA repair helicase RECQ1 has a checkpoint-dependent role in mediating DNA damage responses induced by gemcitabine. J Biol Chem. 2019 10 18; 294(42):15330-15345. PMID: 31444271.
      Citations: 5     Fields:    Translation:HumansCells
    6. Bokhari B, Sharma S. Stress Marks on the Genome: Use or Lose? Int J Mol Sci. 2019 Jan 16; 20(2). PMID: 30654540.
      Citations: 5     Fields:    Translation:HumansAnimalsCells
    7. Li XL, Subramanian M, Jones MF, Chaudhary R, Singh DK, Zong X, Gryder B, Sindri S, Mo M, Schetter A, Wen X, Parvathaneni S, Kazandjian D, Jenkins LM, Tang W, Elloumi F, Martindale JL, Huarte M, Zhu Y, Robles AI, Frier SM, Rigo F, Cam M, Ambs S, Sharma S, Harris CC, Dasso M, Prasanth KV, Lal A. Long Noncoding RNA PURPL Suppresses Basal p53 Levels and Promotes Tumorigenicity in Colorectal Cancer. Cell Rep. 2017 Sep 05; 20(10):2408-2423. PMID: 28877474.
      Citations: 37     Fields:    Translation:HumansCells
    8. Parvathaneni S, Lu X, Chaudhary R, Lal A, Madhusudan S, Sharma S. RECQ1 expression is upregulated in response to DNA damage and in a p53-dependent manner. Oncotarget. 2017 Sep 29; 8(44):75924-75942. PMID: 29100281.
      Citations: 4     Fields:    
    9. Sharma S, Sharma P, Das R, Chhabra S, Hira JK. HbQ-India (HBA1:c.193G>C): hematological profiles and unique CE-HPLC findings of potential diagnostic utility in 65 cases. Ann Hematol. 2017 Jul; 96(7):1227-1229. PMID: 28526955.
      Citations: 2     Fields:    Translation:Humans
    10. Woodrick J, Gupta S, Camacho S, Parvathaneni S, Choudhury S, Cheema A, Bai Y, Khatkar P, Erkizan HV, Sami F, Su Y, Schärer OD, Sharma S, Roy R. A new sub-pathway of long-patch base excision repair involving 5' gap formation. EMBO J. 2017 06 01; 36(11):1605-1622. PMID: 28373211.
      Citations: 18     Fields:    Translation:HumansCells
    11. Sharma S, Dey P, Mitra S, Rajwanshi A, Nijhawan R, Srinivasan R, Gupta N, Das A. Nodular sclerosis classical Hodgkin lymphoma grade 2: A diagnostic challenge to the cytopathologists. Cancer Cytopathol. 2017 Feb; 125(2):104-113. PMID: 27870472.
      Citations: 2     Fields:    Translation:Humans
    12. Arora A, Parvathaneni S, Aleskandarany MA, Agarwal D, Ali R, Abdel-Fatah T, Green AR, Ball GR, Rakha EA, Ellis IO, Sharma S, Madhusudan S. Clinicopathological and Functional Significance of RECQL1 Helicase in Sporadic Breast Cancers. . 2017 01; 16(1):239-250. PMID: 27837030.
    13. Sami F, Gary RK, Fang Y, Sharma S. Site-directed mutants of human RECQ1 reveal functional importance of the zinc binding domain. Mutat Res. 2016 08; 790:8-18. PMID: 27248010.
      Citations: 3     Fields:    Translation:HumansCells
    14. Lu X, Parvathaneni S, Li XL, Lal A, Sharma S. Transcriptome guided identification of novel functions of RECQ1 helicase. Methods. 2016 10 01; 108:111-7. PMID: 27102625.
      Citations: 7     Fields:    Translation:HumansCells
    15. Sami F, Lu X, Parvathaneni S, Roy R, Gary RK, Sharma S. RECQ1 interacts with FEN-1 and promotes binding of FEN-1 to telomeric chromatin. Biochem J. 2015 Jun 01; 468(2):227-44. PMID: 25774876.
      Citations: 9     Fields:    Translation:HumansCells
    16. Sharma S. An appraisal of RECQ1 expression in cancer progression. Front Genet. 2014; 5:426. PMID: 25538733.
    17. Garige M, Sharma S. Cellular deficiency of Werner syndrome protein or RECQ1 promotes genotoxic potential of hydroquinone and benzo[a]pyrene exposure. Int J Toxicol. 2014 Sep-Oct; 33(5):373-81. PMID: 25228686.
      Citations: 3     Fields:    Translation:HumansCells
    18. Li XL, Lu X, Parvathaneni S, Bilke S, Zhang H, Thangavel S, Vindigni A, Hara T, Zhu Y, Meltzer PS, Lal A, Sharma S. Identification of RECQ1-regulated transcriptome uncovers a role of RECQ1 in regulation of cancer cell migration and invasion. . 2014; 13(15):2431-45. PMID: 25483193.
    19. Li XL, Hara T, Choi Y, Subramanian M, Francis P, Bilke S, Walker RL, Pineda M, Zhu Y, Yang Y, Luo J, Wakefield LM, Brabletz T, Park BH, Sharma S, Chowdhury D, Meltzer PS, Lal A. A p21-ZEB1 complex inhibits epithelial-mesenchymal transition through the microRNA 183-96-182 cluster. Mol Cell Biol. 2014 Feb; 34(3):533-50. PMID: 24277930.
      Citations: 44     Fields:    Translation:HumansAnimalsCells
    20. Sami F, Sharma S. Probing Genome Maintenance Functions of human RECQ1. Comput Struct Biotechnol J. 2013; 6:e201303014. PMID: 24688722.
    21. Henderson JT, Puri M, Blum M, Harper CC, Rana A, Gurung G, Pradhan N, Regmi K, Malla K, Sharma S, Grossman D, Bajracharya L, Satyal I, Acharya S, Lamichhane P, Darney PD. Effects of abortion legalization in Nepal, 2001-2010. PLoS One. 2013; 8(5):e64775. PMID: 23741391.
      Citations: 12     Fields:    Translation:Humans
    22. Parvathaneni S, Stortchevoi A, Sommers JA, Brosh RM, Sharma S. Human RECQ1 interacts with Ku70/80 and modulates DNA end-joining of double-strand breaks. PLoS One. 2013; 8(5):e62481. PMID: 23650516.
      Citations: 21     Fields:    Translation:HumansCells
    23. Lu X, Parvathaneni S, Hara T, Lal A, Sharma S. Replication stress induces specific enrichment of RECQ1 at common fragile sites FRA3B and FRA16D. Mol Cancer. 2013 Apr 22; 12(1):29. PMID: 23601052.
      Citations: 23     Fields:    Translation:HumansCells
    24. Sharma S, Phatak P, Stortchevoi A, Jasin M, Larocque JR. RECQ1 plays a distinct role in cellular response to oxidative DNA damage. DNA Repair (Amst). 2012 Jun 01; 11(6):537-49. PMID: 22542292.
      Citations: 26     Fields:    Translation:HumansCells
    25. Sharma S. Non-B DNA Secondary Structures and Their Resolution by RecQ Helicases. J Nucleic Acids. 2011; 2011:724215. PMID: 21977309.
    26. Suhasini AN, Rawtani NA, Wu Y, Sommers JA, Sharma S, Mosedale G, North PS, Cantor SB, Hickson ID, Brosh RM. Interaction between the helicases genetically linked to Fanconi anemia group J and Bloom's syndrome. EMBO J. 2011 Feb 16; 30(4):692-705. PMID: 21240188.
      Citations: 58     Fields:    Translation:HumansAnimalsCells
    27. Sharma S, Brosh RM. Unique and important consequences of RECQ1 deficiency in mammalian cells. . 2008 Apr 15; 7(8):989-1000. PMID: 18414032.
    28. Sharma S, Sommers JA, Brosh RM. Processing of DNA replication and repair intermediates by the concerted action of RecQ helicases and Rad2 structure-specific nucleases. Protein Pept Lett. 2008; 15(1):89-102. PMID: 18221018.
      Citations: 9     Fields:    Translation:HumansCells
    29. Sharma S, Brosh RM. Human RECQ1 is a DNA damage responsive protein required for genotoxic stress resistance and suppression of sister chromatid exchanges. PLoS One. 2007 Dec 12; 2(12):e1297. PMID: 18074021.
      Citations: 57     Fields:    Translation:HumansCells
    30. Gupta R, Sharma S, Sommers JA, Kenny MK, Cantor SB, Brosh RM. FANCJ (BACH1) helicase forms DNA damage inducible foci with replication protein A and interacts physically and functionally with the single-stranded DNA-binding protein. Blood. 2007 Oct 01; 110(7):2390-8. PMID: 17596542.
      Citations: 60     Fields:    Translation:HumansCells
    31. Peng M, Litman R, Xie J, Sharma S, Brosh RM, Cantor SB. The FANCJ/MutLalpha interaction is required for correction of the cross-link response in FA-J cells. EMBO J. 2007 Jul 11; 26(13):3238-49. PMID: 17581638.
      Citations: 93     Fields:    Translation:Cells
    32. Sharma S. Age-related nonhomologous end joining activity in rat neurons. Brain Res Bull. 2007 Jun 15; 73(1-3):48-54. PMID: 17499636.
      Citations: 15     Fields:    Translation:AnimalsCells
    33. Sharma S, Stumpo DJ, Balajee AS, Bock CB, Lansdorp PM, Brosh RM, Blackshear PJ. RECQL, a member of the RecQ family of DNA helicases, suppresses chromosomal instability. Mol Cell Biol. 2007 Mar; 27(5):1784-94. PMID: 17158923.
      Citations: 54     Fields:    Translation:AnimalsCells
    34. Gupta R, Sharma S, Doherty KM, Sommers JA, Cantor SB, Brosh RM. Inhibition of BACH1 (FANCJ) helicase by backbone discontinuity is overcome by increased motor ATPase or length of loading strand. Nucleic Acids Res. 2006; 34(22):6673-83. PMID: 17145708.
      Citations: 26     Fields:    Translation:HumansCells
    35. Sharma S, Doherty KM, Brosh RM. Mechanisms of RecQ helicases in pathways of DNA metabolism and maintenance of genomic stability. Biochem J. 2006 Sep 15; 398(3):319-37. PMID: 16925525.
      Citations: 97     Fields:    Translation:Humans
    36. Doherty KM, Sharma S, Gupta R, Brosh RM. Tetraplex binding molecules as anti-cancer agents. Recent Pat Anticancer Drug Discov. 2006 Jun; 1(2):185-200. PMID: 18221036.
      Citations: 7     Fields:    Translation:HumansAnimalsCells
    37. Muftuoglu M, Sharma S, Thorslund T, Stevnsner T, Soerensen MM, Brosh RM, Bohr VA. Cockayne syndrome group B protein has novel strand annealing and exchange activities. Nucleic Acids Res. 2006; 34(1):295-304. PMID: 16410611.
      Citations: 24     Fields:    Translation:Cells
    38. Brosh RM, Sharma S. Biochemical assays for the characterization of DNA helicases. Methods Mol Biol. 2006; 314:397-415. PMID: 16673896.
      Citations: 3     Fields:    Translation:HumansAnimalsCells
    39. Sharma S, Sommers JA, Gary RK, Friedrich-Heineken E, Hübscher U, Brosh RM. The interaction site of Flap Endonuclease-1 with WRN helicase suggests a coordination of WRN and PCNA. Nucleic Acids Res. 2005; 33(21):6769-81. PMID: 16326861.
      Citations: 31     Fields:    Translation:HumansCells
    40. Sharma S, Sommers JA, Choudhary S, Faulkner JK, Cui S, Andreoli L, Muzzolini L, Vindigni A, Brosh RM. Biochemical analysis of the DNA unwinding and strand annealing activities catalyzed by human RECQ1. J Biol Chem. 2005 Jul 29; 280(30):28072-84. PMID: 15899892.
      Citations: 93     Fields:    Translation:HumansCells
    41. Doherty KM, Sharma S, Uzdilla LA, Wilson TM, Cui S, Vindigni A, Brosh RM. RECQ1 helicase interacts with human mismatch repair factors that regulate genetic recombination. J Biol Chem. 2005 Jul 29; 280(30):28085-94. PMID: 15886194.
      Citations: 45     Fields:    Translation:HumansCells
    42. Gupta R, Sharma S, Sommers JA, Jin Z, Cantor SB, Brosh RM. Analysis of the DNA substrate specificity of the human BACH1 helicase associated with breast cancer. J Biol Chem. 2005 Jul 08; 280(27):25450-60. PMID: 15878853.
      Citations: 65     Fields:    Translation:HumansCells
    43. Sharma S, Doherty KM, Brosh RM. DNA helicases as targets for anti-cancer drugs. Curr Med Chem Anticancer Agents. 2005 May; 5(3):183-99. PMID: 15992349.
      Citations: 22     Fields:    Translation:HumansAnimalsCells
    44. Sommers JA, Sharma S, Doherty KM, Karmakar P, Yang Q, Kenny MK, Harris CC, Brosh RM. p53 modulates RPA-dependent and RPA-independent WRN helicase activity. Cancer Res. 2005 Feb 15; 65(4):1223-33. PMID: 15735006.
      Citations: 21     Fields:    Translation:HumansCells
    45. Sharma S, Sommers JA, Brosh RM. In vivo function of the conserved non-catalytic domain of Werner syndrome helicase in DNA replication. Hum Mol Genet. 2004 Oct 01; 13(19):2247-61. PMID: 15282207.
      Citations: 28     Fields:    Translation:HumansAnimalsCells
    46. Sharma S, Sommers JA, Wu L, Bohr VA, Hickson ID, Brosh RM. Stimulation of flap endonuclease-1 by the Bloom's syndrome protein. J Biol Chem. 2004 Mar 12; 279(11):9847-56. PMID: 14688284.
      Citations: 52     Fields:    Translation:HumansAnimalsCells
    47. Sharma S, Otterlei M, Sommers JA, Driscoll HC, Dianov GL, Kao HI, Bambara RA, Brosh RM. WRN helicase and FEN-1 form a complex upon replication arrest and together process branchmigrating DNA structures associated with the replication fork. Mol Biol Cell. 2004 Feb; 15(2):734-50. PMID: 14657243.
      Citations: 63     Fields:    Translation:HumansCells
    48. Sharma S, Sommers JA, Driscoll HC, Uzdilla L, Wilson TM, Brosh RM. The exonucleolytic and endonucleolytic cleavage activities of human exonuclease 1 are stimulated by an interaction with the carboxyl-terminal region of the Werner syndrome protein. J Biol Chem. 2003 Jun 27; 278(26):23487-96. PMID: 12704184.
      Citations: 30     Fields:    Translation:HumansCells
    49. Rathaur S, Sharma S, Singh RN, Henkle K, Selkirk ME. Antibody responses of Wuchereria bancrofti patients to recombinant Brugia pahangi superoxide dismutase. Indian J Exp Biol. 2001 Jan; 39(1):35-40. PMID: 11349523.
      Citations:    Fields:    Translation:HumansAnimals
    50. Sharma S, Sharma M, Rathaur S. Bancroftian filariasis in the Varanasi region of north India: an epidemiological study. Ann Trop Med Parasitol. 1999 Jun; 93(4):379-87. PMID: 10656039.
      Citations: 2     Fields:    Translation:HumansAnimals
    51. Sharma S, Rathaur S. Characterization of secretory acetylcholinesterase from Setaria cervi microfilariae: a potential antigen for diagnosis of human filariasis. Trop Med Int Health. 1999 May; 4(5):341-8. PMID: 10402969.
      Citations:    Fields:    Translation:HumansAnimals
    52. Sharma S, Misra S, Rathaur S. Secretory acetylcholinesterase of Setaria cervi microfilariae and its antigenic cross-reactivity with Wuchereria bancrofti. Trop Med Int Health. 1998 Jan; 3(1):46-51. PMID: 9484968.
      Citations: 2     Fields:    Translation:HumansAnimals
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