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Chiung-Yu Hung
Title Associate Professor
Faculty Rank
Degree Ph.D.
Institution University of Texas at San Antonio
Department Biology
Clusters Other
Address
1 UTSA Cir
City San Antonio
State TX
Postal Code 78249
Telephone (210) 458-7018
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Title Immune Defense Core (Flow)
Institution University of Texas at San Antonio
Department Biology

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  My research is focused on studies of virulence factors of Coccidioides spp. which are causative agents of coccidioidomycosis. Coccidioides can cause a respiratory disease in healthy individuals as well as immunocompromised patients. In the United States there is an estimated 150,000 yearly cases of coccidioidal infection and it is considered to be a reemerging infectious disease. My research interest is in the identification and characterization of dominant antigens of Coccidioides spp. which elicit innate inflammatory response or stimulate acquired immunity to infection with the fungal pathogen. Studies of the interaction between the host and these antigens may lead to a better understanding of the course of disease, and to the identification of vaccine candidates or novel therapeutic drug targets. I am also interested in the application of biochemical, molecular, genetic, and bioinformatic techniques to the characterization of proteins involved in cell wall synthesis and degradation. Coccidioides spp. is a diphasic fungus characterized by a parasitic cycle (Fig.1) which is unique amongst medically important fungal pathogens. Cell wall synthases, hydrolases and their regulatory proteins have been proposed to play key roles in morphogenesis and, therefore, represent potential molecular targets for novel antifungal drugs.

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  Research projects funded by the National Institutes of Health (NIH), the Centers for Disease Control (CDC), the Food and Drug Administration (FDA), and the Department of Veterans Affairs (VA)

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1.
2018Development of a multivalent vaccine against Coccidioides infection1R01AI135005-01A1
2.
2016Enhancement of protective efficacy of Coccidioides vaccines by adjuvants5R21AI114762-02
3.
2015Enhancement of protective efficacy of Coccidioides vaccines by adjuvants1R21AI114762-01A1

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1. Trivedi NH, Yu JJ, Hung CY, Doelger RP, Navara CS, Armitige LY, Seshu J, Sinai AP, Chambers JP, Guentzel MN, Arulanandam BP. Microbial co-infection alters macrophage polarization, phagosomal escape, and microbial killing. Innate Immun. 2018 Apr; 24(3):152-162.
2. Castro-Lopez N, Hung CY. Immune Response to Coccidioidomycosis and the Development of a Vaccine. Microorganisms. 2017 Mar 16; 5(1).
3. Hurtgen BJ, Hung CY. Rational Design of T Lymphocyte Epitope-Based Vaccines Against Coccidioides Infection. Methods Mol Biol. 2017; 1625:45-64.
4. Hurtgen BJ, Castro-Lopez N, Jiménez-Alzate MDP, Cole GT, Hung CY. Preclinical identification of vaccine induced protective correlates in human leukocyte antigen expressing transgenic mice infected with Coccidioides posadasii. Vaccine. 2016 10 17; 34(44):5336-5343.
5. Wang H, Li M, Hung CY, Sinha M, Lee LM, Wiesner DL, LeBert V, Lerksuthirat T, Galles K, Suresh M, DeFranco AL, Lowell CA, Klein BS, Wüthrich M. MyD88 Shapes Vaccine Immunity by Extrinsically Regulating Survival of CD4+ T Cells during the Contraction Phase. PLoS Pathog. 2016 Aug; 12(8):e1005787.
6. Cunningham AL, Guentzel MN, Yu JJ, Hung CY, Forsthuber TG, Navara CS, Yagita H, Williams IR, Klose KE, Eaves-Pyles TD, Arulanandam BP. M-Cells Contribute to the Entry of an Oral Vaccine but Are Not Essential for the Subsequent Induction of Protective Immunity against Francisella tularensis. PLoS One. 2016; 11(4):e0153402.
7. Hung CY, Castro-Lopez N, Cole GT. Card9- and MyD88-Mediated Gamma Interferon and Nitric Oxide Production Is Essential for Resistance to Subcutaneous Coccidioides posadasii Infection. Infect Immun. 2016 Apr; 84(4):1166-75.
8. Wüthrich M, Gern B, Hung CY, Ersland K, Rocco N, Pick-Jacobs J, Galles K, Filutowicz H, Warner T, Evans M, Cole G, Klein B. Vaccine-induced protection against 3 systemic mycoses endemic to North America requires Th17 cells in mice. J Clin Invest. 2016 Feb; 126(2):795.
9. Hung CY, Wozniak KL, Cole GT. Flow Cytometric Analysis of Protective T-Cell Response Against Pulmonary Coccidioides Infection. Methods Mol Biol. 2016; 1403:551-66.
10. Wüthrich M, Brandhorst TT, Sullivan TD, Filutowicz H, Sterkel A, Stewart D, Li M, Lerksuthirat T, LeBert V, Shen ZT, Ostroff G, Deepe GS, Hung CY, Cole G, Walter JA, Jenkins MK, Klein B. Calnexin induces expansion of antigen-specific CD4(+) T cells that confer immunity to fungal ascomycetes via conserved epitopes. Cell Host Microbe. 2015 Apr 08; 17(4):452-65.
11. Kittur FS, Lalgondar M, Hung CY, Sane DC, Xie J. C-Terminally fused affinity Strep-tag II is removed by proteolysis from recombinant human erythropoietin expressed in transgenic tobacco plants. Plant Cell Rep. 2015 Mar; 34(3):507-16.
12. Hung CY, Aspesi P, Hunter MR, Lomax AW, Perera IY. Phosphoinositide-signaling is one component of a robust plant defense response. Front Plant Sci. 2014; 5:267.
13. Hung CY, Jiménez-Alzate Mdel P, Gonzalez A, Wüthrich M, Klein BS, Cole GT. Interleukin-1 receptor but not Toll-like receptor 2 is essential for MyD88-dependent Th17 immunity to Coccidioides infection. Infect Immun. 2014 May; 82(5):2106-14.
14. Wang H, LeBert V, Hung CY, Galles K, Saijo S, Lin X, Cole GT, Klein BS, Wüthrich M. C-type lectin receptors differentially induce th17 cells and vaccine immunity to the endemic mycosis of North America. J Immunol. 2014 Feb 01; 192(3):1107-1119.
15. Cole GT, Hung CY, Sanderson SD, Hurtgen BJ, Wüthrich M, Klein BS, Deepe GS, Ostroff GR, Levitz SM. Novel strategies to enhance vaccine immunity against coccidioidomycosis. PLoS Pathog. 2013; 9(12):e1003768.
16. Hung CY, Castro-Lopez N, Cole GT. Vaccinated C57BL/6 mice develop protective and memory T cell responses to Coccidioides posadasii infection in the absence of interleukin-10. Infect Immun. 2014 Feb; 82(2):903-13.
17. Kittur FS, Bah M, Archer-Hartmann S, Hung CY, Azadi P, Ishihara M, Sane DC, Xie J. Cytoprotective effect of recombinant human erythropoietin produced in transgenic tobacco plants. PLoS One. 2013; 8(10):e76468.
18. Wise HZ, Hung CY, Whiston E, Taylor JW, Cole GT. Extracellular ammonia at sites of pulmonary infection with Coccidioides posadasii contributes to severity of the respiratory disease. Microb Pathog. 2013 Jun-Jul; 59-60:19-28.
19. Hung CY, Fan L, Kittur FS, Sun K, Qiu J, Tang S, Holliday BM, Xiao B, Burkey KO, Bush LP, Conkling MA, Roje S, Xie J. Alteration of the alkaloid profile in genetically modified tobacco reveals a role of methylenetetrahydrofolate reductase in nicotine N-demethylation. Plant Physiol. 2013 Feb; 161(2):1049-60.
20. Cole GT, Hurtgen BJ, Hung CY. Progress Toward a Human Vaccine Against Coccidioidomycosis. Curr Fungal Infect Rep. 2012 Dec 01; 6(4):235-244.
21. Hurtgen BJ, Hung CY, Ostroff GR, Levitz SM, Cole GT. Construction and evaluation of a novel recombinant T cell epitope-based vaccine against Coccidioidomycosis. Infect Immun. 2012 Nov; 80(11):3960-74.
22. Mgbemena V, Segovia JA, Chang TH, Tsai SY, Cole GT, Hung CY, Bose S. Transactivation of inducible nitric oxide synthase gene by Kruppel-like factor 6 regulates apoptosis during influenza A virus infection. J Immunol. 2012 Jul 15; 189(2):606-15.
23. Hung CY, Hurtgen BJ, Bellecourt M, Sanderson SD, Morgan EL, Cole GT. An agonist of human complement fragment C5a enhances vaccine immunity against Coccidioides infection. Vaccine. 2012 Jun 29; 30(31):4681-90.
24. Hung CY, Wise HZ, Cole GT. Gene disruption in Coccidioides using hygromycin or phleomycin resistance markers. Methods Mol Biol. 2012; 845:131-47.
25. Gonzalez A, Hung CY, Cole GT. Absence of phagocyte NADPH oxidase 2 leads to severe inflammatory response in lungs of mice infected with Coccidioides. Microb Pathog. 2011 Dec; 51(6):432-41.
26. Hung CY, Gonzalez A, Wüthrich M, Klein BS, Cole GT. Vaccine immunity to coccidioidomycosis occurs by early activation of three signal pathways of T helper cell response (Th1, Th2, and Th17). Infect Immun. 2011 Nov; 79(11):4511-22.
27. Wüthrich M, Hung CY, Gern BH, Pick-Jacobs JC, Galles KJ, Filutowicz HI, Cole GT, Klein BS. A TCR transgenic mouse reactive with multiple systemic dimorphic fungi. J Immunol. 2011 Aug 01; 187(3):1421-31.
28. Gonzalez A, Hung CY, Cole GT. Nitric oxide synthase activity has limited influence on the control of Coccidioides infection in mice. Microb Pathog. 2011 Sep; 51(3):161-8.
29. Wüthrich M, Gern B, Hung CY, Ersland K, Rocco N, Pick-Jacobs J, Galles K, Filutowicz H, Warner T, Evans M, Cole G, Klein B. Vaccine-induced protection against 3 systemic mycoses endemic to North America requires Th17 cells in mice. J Clin Invest. 2011 Feb; 121(2):554-68.
30. Gonzalez A, Hung CY, Cole GT. Coccidioides releases a soluble factor that suppresses nitric oxide production by murine primary macrophages. Microb Pathog. 2011 Feb; 50(2):100-8.
31. Neafsey DE, Barker BM, Sharpton TJ, Stajich JE, Park DJ, Whiston E, Hung CY, McMahan C, White J, Sykes S, Heiman D, Young S, Zeng Q, Abouelleil A, Aftuck L, Bessette D, Brown A, FitzGerald M, Lui A, Macdonald JP, Priest M, Orbach MJ, Galgiani JN, Kirkland TN, Cole GT, Birren BW, Henn MR, Taylor JW, Rounsley SD. Population genomic sequencing of Coccidioides fungi reveals recent hybridization and transposon control. Genome Res. 2010 Jul; 20(7):938-46.
32. Hung CY, Sun YH, Chen J, Darlington DE, Williams AL, Burkey KO, Xie J. Identification of a Mg-protoporphyrin IX monomethyl ester cyclase homologue, EaZIP, differentially expressed in variegated Epipremnum aureum 'Golden Pothos' is achieved through a unique method of comparative study using tissue regenerated plants. J Exp Bot. 2010 Mar; 61(5):1483-93.
33. Phillips JA, Morgan EL, Dong Y, Cole GT, McMahan C, Hung CY, Sanderson SD. Single-step conjugation of bioactive peptides to proteins via a self-contained succinimidyl bis-arylhydrazone. Bioconjug Chem. 2009 Oct 21; 20(10):1950-7.
34. Sharpton TJ, Stajich JE, Rounsley SD, Gardner MJ, Wortman JR, Jordar VS, Maiti R, Kodira CD, Neafsey DE, Zeng Q, Hung CY, McMahan C, Muszewska A, Grynberg M, Mandel MA, Kellner EM, Barker BM, Galgiani JN, Orbach MJ, Kirkland TN, Cole GT, Henn MR, Birren BW, Taylor JW. Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives. Genome Res. 2009 Oct; 19(10):1722-31.
35. Xue J, Chen X, Selby D, Hung CY, Yu JJ, Cole GT. A genetically engineered live attenuated vaccine of Coccidioides posadasii protects BALB/c mice against coccidioidomycosis. Infect Immun. 2009 Aug; 77(8):3196-208.
36. Herr RA, Hung CY, Cole GT. Evaluation of two homologous proline-rich proteins of Coccidioides posadasii as candidate vaccines against coccidioidomycosis. Infect Immun. 2007 Dec; 75(12):5777-87.
37. Hung CY, Xue J, Cole GT. Virulence mechanisms of coccidioides. Ann N Y Acad Sci. 2007 Sep; 1111:225-35.
38. Nosanchuk JD, Yu JJ, Hung CY, Casadevall A, Cole GT. Coccidioides posadasii produces melanin in vitro and during infection. Fungal Genet Biol. 2007 Jun; 44(6):517-20.
39. Tarcha EJ, Basrur V, Hung CY, Gardner MJ, Cole GT. Multivalent recombinant protein vaccine against coccidioidomycosis. Infect Immun. 2006 Oct; 74(10):5802-13.
40. Shubitz LF, Yu JJ, Hung CY, Kirkland TN, Peng T, Perrill R, Simons J, Xue J, Herr RA, Cole GT, Galgiani JN. Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein. Vaccine. 2006 Jul 26; 24(31-32):5904-11.
41. Mirbod-Donovan F, Schaller R, Hung CY, Xue J, Reichard U, Cole GT. Urease produced by Coccidioides posadasii contributes to the virulence of this respiratory pathogen. Infect Immun. 2006 Jan; 74(1):504-15.
42. Tarcha EJ, Basrur V, Hung CY, Gardner MJ, Cole GT. A recombinant aspartyl protease of Coccidioides posadasii induces protection against pulmonary coccidioidomycosis in mice. Infect Immun. 2006 Jan; 74(1):516-27.
43. Yu JJ, Kirkland TN, Hall LK, Wopschall J, Smith RC, Hung CY, Chen X, Tarcha E, Thomas PW, Cole GT. Characterization of a serodiagnostic complement fixation antigen of Coccidioides posadasii expressed in the nonpathogenic Fungus Uncinocarpus reesii. J Clin Microbiol. 2005 Nov; 43(11):5462-9.
44. Hung CY, Seshan KR, Yu JJ, Schaller R, Xue J, Basrur V, Gardner MJ, Cole GT. A metalloproteinase of Coccidioides posadasii contributes to evasion of host detection. Infect Immun. 2005 Oct; 73(10):6689-703.
45. Johannesson H, Townsend JP, Hung CY, Cole GT, Taylor JW. Concerted evolution in the repeats of an immunomodulating cell surface protein, SOWgp, of the human pathogenic fungi Coccidioides immitis and C. posadasii. Genetics. 2005 Sep; 171(1):109-17.
46. Xue J, Hung CY, Yu JJ, Cole GT. Immune response of vaccinated and non-vaccinated mice to Coccidioides posadasii infection. Vaccine. 2005 May 20; 23(27):3535-44.
47. Cole GT, Xue JM, Okeke CN, Tarcha EJ, Basrur V, Schaller RA, Herr RA, Yu JJ, Hung CY. A vaccine against coccidioidomycosis is justified and attainable. Med Mycol. 2004 Jun; 42(3):189-216.
48. Delgado N, Hung CY, Tarcha E, Gardner MJ, Cole GT. Profiling gene expression in Coccidioides posadasii. Med Mycol. 2004 Feb; 42(1):59-71.
49. Delgado N, Xue J, Yu JJ, Hung CY, Cole GT. A recombinant beta-1,3-glucanosyltransferase homolog of Coccidioides posadasii protects mice against coccidioidomycosis. Infect Immun. 2003 Jun; 71(6):3010-9.
50. Hung CY, Yu JJ, Seshan KR, Reichard U, Cole GT. A parasitic phase-specific adhesin of Coccidioides immitis contributes to the virulence of this respiratory Fungal pathogen. Infect Immun. 2002 Jul; 70(7):3443-56.
51. Li K, Yu JJ, Hung CY, Lehmann PF, Cole GT. Recombinant urease and urease DNA of Coccidioides immitis elicit an immunoprotective response against coccidioidomycosis in mice. Infect Immun. 2001 May; 69(5):2878-87.
52. Hung CY, Yu JJ, Lehmann PF, Cole GT. Cloning and expression of the gene which encodes a tube precipitin antigen and wall-associated beta-glucosidase of Coccidioides immitis. Infect Immun. 2001 Apr; 69(4):2211-22.
53. Cole GT, Hung CY. The parasitic cell wall of Coccidioides immitis. Med Mycol. 2001; 39 Suppl 1:31-40.
54. Reichard U, Hung CY, Thomas PW, Cole GT. Disruption of the gene which encodes a serodiagnostic antigen and chitinase of the human fungal pathogen Coccidioides immitis. Infect Immun. 2000 Oct; 68(10):5830-8.
55. Hung CY, Ampel NM, Christian L, Seshan KR, Cole GT. A major cell surface antigen of Coccidioides immitis which elicits both humoral and cellular immune responses. Infect Immun. 2000 Feb; 68(2):584-93.
56. Guevara-Olvera L, Hung CY, Yu JJ, Cole GT. Sequence, expression and functional analysis of the Coccidioides immitis ODC (ornithine decarboxylase) gene. Gene. 2000 Jan 25; 242(1-2):437-48.

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