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Qiyi Tang
Title Dr
Faculty Rank Associate Professor
Degree PhD
Institution Howard University
Department Microbiology
Clusters Cancer
HIV/AIDS
Infectious and Immunological Diseases
Address
520 W Street NW
City Washington
State DC
Postal Code 20059
Telephone 2028063915
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Title Associate Professor
Institution Howard University, College of Medicine
Department Microbiology
Division Virology

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  A. Research In Cytomegaloviruses (CMV). My studies have been focused on CMV gene regulation at the very early time of infection. During the immediate early (IE) stage of CMV infection, the cell uses widely different cellular proteins, in its defensive arsenal, such as nuclear domains 10 (ND10) components (Daxx, PML and SP100), nuclear suppressors (HDAC), apoptotic pathway molecules. These proteins suppress major immediate early promoter (MIEP) activity. Viruses have also evolved molecular anti-defense mechanisms. For example, CMV gene products IE2 can shut off cellular activities so that the virus can usurp cellular machineries for viral gene expression and DNA replication; IE1 disperses PML bodies and represses HDAC activity. We have recently mapped out a small domain of IE1 be responsible for murine CMV to disperse ND10. MIE gene products (IE1 and IE2 for HCMV or IE3 for MCMV) are then responsible for activation of early gene. IE3 of MCMV (homology of IE2 of HCMV) is a suppressor of MIEP and activator of early gene (e.g. 112-113 gene). IE3’s suppressive effects on MIEP can be eliminated by 112-113 gene products. IE3 can also interact with HDAC to reduce the HDAC activity, which play a role in activating early gene promoter. We very recently mapped out a small motif in 112-113 gene promoter region, and it is called IE3BAM that is essential for IE3 to activate 112-113 gene expression. We also found that IE3 activates 112-113 gene promoter via interaction with TBP to stabilize TFIID complexes. These studies will advance our understanding of the mechanisms of CMV latency and reactivation and may lead to the development of new therapies to prevent CMV-caused disease.

B. Research In Kaposi’s Sarcoma Associated Herpesvirus (KSHV). KSHV (also known as Human herpesvirus 8) has been determined to be the most frequent cause of malignancies in AIDS patients. It is associated primarily with Kaposi’s sarcoma and primary effusion lymphoma (PEL), as well as with multicentric Castleman’s disease (MCD). The switch from the latent to the lytic stage is important both in maintenance of malignancy and viral infection. Therefore, strategies for the treatment of KSHV-related malignancies need to both prevent cellular proliferation and block viral production. Only a few genes can be expressed during latency, and these gene products tether KSHV DNA episomes with chromosomes in order to keep KSHV in its latent state. Several chemicals, including 12-O-Tetradecanoyl-phorbol-13-acetate (TPA), sodium butyrate (NaB), and 5-azacytidin e (5-AC), can reactivate KSHV from latency in cell cultures. RTA (also called ORF50) gene expression is the switch point from latency to the lytic cycle because RTA is essential and sufficient for the reactivation of KSHV, but the pathological mechanism of the reactivation of KSHV is poorly understood. Prior studies on the reactivation of KSHV using chemical inducers implied that epigenetic modification, especially chromatin remodeling by acetylation, is critical for transactivators to access lytic gene promoters. Our studies also indicate that another KSHV-encoded protein, K-bZIP, is critical in reactivation of KSHV. We mapped out that the leucine zipper domain is essential for K-bZIP to interact with HDAC and reduce HDAC activity, which function play a role in viral replication.

NIH Awarded Grants icon

  Research projects funded by the National Institutes of Health (NIH), the Centers for Disease Control (CDC), the Food and Drug Administration (FDA), and the Department of Veterans Affairs (VA)

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1.
2017In vitro and in vivo studies of Cytomegalovirus MIE gene regulation5SC1AI112785-04
2.
2016In vitro and in vivo studies of Cytomegalovirus MIE gene regulation5SC1AI112785-03
3.
2015In vitro and in vivo studies of Cytomegalovirus MIE gene regulation7SC1AI112785-02
4.
2014In vitro and in vivo studies of Cytomegalovirus MIE gene regulation1SC1AI112785-01

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1. Yang B, Liu XJ, Yao Y, Jiang X, Wang XZ, Yang H, Sun JY, Miao Y, Wang W, Huang ZL, Wang Y, Tang Q, Rayner S, Britt WJ, McVoy MA, Luo MH, Zhao F. WDR5 Facilitates Human Cytomegalovirus Replication by Promoting Capsid Nuclear Egress. J Virol. 2018 May 01; 92(9).
2. Yang L, Wang R, Yang S, Ma Z, Lin S, Nan Y, Li Q, Tang Q, Zhang YJ. Karyopherin Alpha 6 Is Required for Replication of Porcine Reproductive and Respiratory Syndrome Virus and Zika Virus. J Virol. 2018 May 01; 92(9).
3. Li S, Armstrong N, Zhao H, Hou W, Liu J, Chen C, Wan J, Wang W, Zhong C, Liu C, Zhu H, Xia N, Cheng T, Tang Q. Zika Virus Fatally Infects Wild Type Neonatal Mice and Replicates in Central Nervous System. Viruses. 2018 01 22; 10(1).
4. Zhu R, Cheng T, Yin Z, Liu D, Xu L, Li Y, Wang W, Liu J, Que Y, Ye X, Tang Q, Zhao Q, Ge S, He S, Xia N. Serological survey of neutralizing antibodies to eight major enteroviruses among healthy population. Emerg Microbes Infect. 2018 Jan 10; 7(1):2.
5. Perez KJ, Martínez FP, Cosme-Cruz R, Perez-Crespo NM, Tang Q. Correction for Perez et al., "A Short cis-Acting Motif in the M112-113 Promoter Region Is Essential for IE3 To Activate M112-113 Gene Expression and Is Important for Murine Cytomegalovirus Replication". J Virol. 2017 12 01; 91(23).
6. Liu XJ, Yang B, Huang SN, Wu CC, Li XJ, Cheng S, Jiang X, Hu F, Ming YZ, Nevels M, Britt WJ, Rayner S, Tang Q, Zeng WB, Zhao F, Luo MH. Human cytomegalovirus IE1 downregulates Hes1 in neural progenitor cells as a potential E3 ubiquitin ligase. PLoS Pathog. 2017 Jul; 13(7):e1006542.
7. Cheng S, Jiang X, Yang B, Wen L, Zhao F, Zeng WB, Liu XJ, Dong X, Sun JY, Ming YZ, Zhu H, Rayner S, Tang Q, Fortunato E, Luo MH. Infected T98G glioblastoma cells support human cytomegalovirus reactivation from latency. Virology. 2017 10; 510:205-215.
8. Hou W, Cruz-Cosme R, Armstrong N, Obwolo LA, Wen F, Hu W, Luo MH, Tang Q. Molecular cloning and characterization of the genes encoding the proteins of Zika virus. Gene. 2017 Sep 10; 628:117-128.
9. Hou W, Armstrong N, Obwolo LA, Thomas M, Pang X, Jones KS, Tang Q. Determination of the Cell Permissiveness Spectrum, Mode of RNA Replication, and RNA-Protein Interaction of Zika Virus. BMC Infect Dis. 2017 03 31; 17(1):239.
10. Armstrong N, Hou W, Tang Q. Biological and historical overview of Zika virus. World J Virol. 2017 Feb 12; 6(1):1-8.
11. Hou W, Torres L, Cruz-Cosme R, Arroyo F, Irizarry L, Luciano D, Márquez A, Rivera LL, Sala AL, Luo MH, Tang Q. Two Polypyrimidine Tracts in Intron 4 of the Major Immediate Early Gene Are Critical for Gene Expression Switching from IE1 to IE2 and for Replication of Human Cytomegalovirus. J Virol. 2016 08 15; 90(16):7339-7349.
12. Tankou S, Ishii K, Elliott C, Yalla KC, Day JP, Furukori K, Kubo KI, Brandon NJ, Tang Q, Hayward G, Nakajima K, Houslay MD, Kamiya A, Baillie G, Ishizuka K, Sawa A. SUMOylation of DISC1: a potential role in neural progenitor proliferation in the developing cortex. Mol Neuropsychiatry. 2016 May; 2(1):20-27.
13. Li XJ, Liu XJ, Yang B, Fu YR, Zhao F, Shen ZZ, Miao LF, Rayner S, Chavanas S, Zhu H, Britt WJ, Tang Q, McVoy MA, Luo MH. Human Cytomegalovirus Infection Dysregulates the Localization and Stability of NICD1 and Jag1 in Neural Progenitor Cells. J Virol. 2015 Jul; 89(13):6792-804.
14. Torres L, Ortiz T, Tang Q. Enhancement of herpes simplex virus (HSV) infection by seminal plasma and semen amyloids implicates a new target for the prevention of HSV infection. Viruses. 2015 Apr 20; 7(4):2057-73.
15. Torres L, Tang Q. Immediate-Early (IE) gene regulation of cytomegalovirus: IE1- and pp71-mediated viral strategies against cellular defenses. Virol Sin. 2014 Dec; 29(6):343-52.
16. Fu YR, Liu XJ, Li XJ, Shen ZZ, Yang B, Wu CC, Li JF, Miao LF, Ye HQ, Qiao GH, Rayner S, Chavanas S, Davrinche C, Britt WJ, Tang Q, McVoy M, Mocarski E, Luo MH. MicroRNA miR-21 attenuates human cytomegalovirus replication in neural cells by targeting Cdc25a. J Virol. 2015 Jan 15; 89(2):1070-82.
17. Martínez FP, Cruz R, Lu F, Plasschaert R, Deng Z, Rivera-Molina YA, Bartolomei MS, Lieberman PM, Tang Q. CTCF binding to the first intron of the major immediate early (MIE) gene of human cytomegalovirus (HCMV) negatively regulates MIE gene expression and HCMV replication. J Virol. 2014 Jul; 88(13):7389-401.
18. Martínez FP, Tang Q. Identification of cellular proteins that interact with human cytomegalovirus immediate-early protein 1 by protein array assay. Viruses. 2013 Dec 31; 6(1):89-105.
19. Tang Q, Roan NR, Yamamura Y. Seminal plasma and semen amyloids enhance cytomegalovirus infection in cell culture. J Virol. 2013 Dec; 87(23):12583-91.
20. Hui WH, Tang Q, Liu H, Atanasov I, Liu F, Zhu H, Zhou ZH. Protein interactions in the murine cytomegalovirus capsid revealed by cryoEM. Protein Cell. 2013 Nov; 4(11):833-45.
21. Rivera-Molina YA, Martínez FP, Tang Q. Nuclear domain 10 of the viral aspect. World J Virol. 2013 Aug 12; 2(3):110-22.
22. Perez KJ, Martínez FP, Cosme-Cruz R, Perez-Crespo NM, Tang Q. A short cis-acting motif in the M112-113 promoter region is essential for IE3 to activate M112-113 gene expression and is important for murine cytomegalovirus replication. J Virol. 2013 Mar; 87(5):2639-47.
23. Rivera-Molina YA, Rojas BR, Tang Q. Nuclear domain 10-associated proteins recognize and segregate intranuclear DNA/protein complexes to negate gene expression. Virol J. 2012 Sep 28; 9:222.
24. Selariu A, Cheng T, Tang Q, Silver B, Yang L, Liu C, Ye X, Markus A, Goldstein RS, Cruz-Cosme RS, Lin Y, Wen L, Qian H, Han J, Dulal K, Huang Y, Li Y, Xia N, Zhu H. ORF7 of varicella-zoster virus is a neurotropic factor. J Virol. 2012 Aug; 86(16):8614-24.
25. Martínez FP, Tang Q. Leucine zipper domain is required for Kaposi sarcoma-associated herpesvirus (KSHV) K-bZIP protein to interact with histone deacetylase and is important for KSHV replication. J Biol Chem. 2012 May 04; 287(19):15622-34.
26. Liang Q, Deng H, Li X, Wu X, Tang Q, Chang TH, Peng H, Rauscher FJ, Ozato K, Zhu F. Tripartite motif-containing protein 28 is a small ubiquitin-related modifier E3 ligase and negative regulator of IFN regulatory factor 7. J Immunol. 2011 Nov 01; 187(9):4754-63.
27. Cosme-Cruz R, Martínez FP, Perez KJ, Tang Q. H2B homology region of major immediate-early protein 1 is essential for murine cytomegalovirus to disrupt nuclear domain 10, but is not important for viral replication in cell culture. J Gen Virol. 2011 Sep; 92(Pt 9):2006-19.
28. Cosme RC, Martínez FP, Tang Q. Functional interaction of nuclear domain 10 and its components with cytomegalovirus after infections: cross-species host cells versus native cells. PLoS One. 2011 Apr 28; 6(4):e19187.
29. Huang Y, Tang Q, Nguyen M, Dulal K, Wang W, Zhu H. Histone deacetylase 3, not histone deacetylase 2, interacts with the major immediate early locus of human cytomegalovirus. Virol J. 2011 Mar 31; 8:151.
30. Warden C, Tang Q, Zhu H. Herpesvirus BACs: past, present, and future. J Biomed Biotechnol. 2011; 2011:124595.
31. Martínez FP, Cosme RS, Tang Q. Murine cytomegalovirus major immediate-early protein 3 interacts with cellular and viral proteins in viral DNA replication compartments and is important for early gene activation. J Gen Virol. 2010 Nov; 91(Pt 11):2664-76.
32. Cheng B, Martínez FP, Katano H, Tang Q. Evidence of inability of human cytomegalovirus to reactivate Kaposi's sarcoma-associated herpesvirus from latency in body cavity-based lymphocytes. J Clin Virol. 2009 Nov; 46(3):244-8.
33. Hanson LK, Slater JS, Cavanaugh VJ, Newcomb WW, Bolin LL, Nelson CN, Fetters LD, Tang Q, Brown JC, Maul GG, Campbell AE. Murine cytomegalovirus capsid assembly is dependent on US22 family gene M140 in infected macrophages. J Virol. 2009 Aug; 83(15):7449-56.
34. Cosme RS, Yamamura Y, Tang Q. Roles of polypyrimidine tract binding proteins in major immediate-early gene expression and viral replication of human cytomegalovirus. J Virol. 2009 Apr; 83(7):2839-50.
35. Wang Y, Li H, Tang Q, Maul GG, Yuan Y. Kaposi's sarcoma-associated herpesvirus ori-Lyt-dependent DNA replication: involvement of host cellular factors. J Virol. 2008 Mar; 82(6):2867-82.
36. Kuang E, Tang Q, Maul GG, Zhu F. Activation of p90 ribosomal S6 kinase by ORF45 of Kaposi's sarcoma-associated herpesvirus and its role in viral lytic replication. J Virol. 2008 Feb; 82(4):1838-50.
37. Wang Y, Tang Q, Maul GG, Yuan Y. Kaposi's sarcoma-associated herpesvirus ori-Lyt-dependent DNA replication: dual role of replication and transcription activator. J Virol. 2006 Dec; 80(24):12171-86.
38. Su YH, Zhang X, Aiamkitsumrit B, Tang Q, Maul G, Fraser NW, Block TM. The stability of herpes simplex virus type I genomes in infected Vero cells undergoing viral induced apoptosis. J Neurovirol. 2006 Oct; 12(5):375-86.
39. Tang Q, Maul GG. Mouse cytomegalovirus crosses the species barrier with help from a few human cytomegalovirus proteins. J Virol. 2006 Aug; 80(15):7510-21.
40. Tang Q, Murphy EA, Maul GG. Experimental confirmation of global murine cytomegalovirus open reading frames by transcriptional detection and partial characterization of newly described gene products. J Virol. 2006 Jul; 80(14):6873-82.
41. Vanniasinkam T, Ertl H, Tang Q. Trichostatin-A enhances adaptive immune responses to DNA vaccination. J Clin Virol. 2006 Aug; 36(4):292-7.
42. Tang Q, Li L, Maul GG. Mouse cytomegalovirus early M112/113 proteins control the repressive effect of IE3 on the major immediate-early promoter. J Virol. 2005 Jan; 79(1):257-63.
43. Tang Q, Li L, Ishov AM, Revol V, Epstein AL, Maul GG. Determination of minimum herpes simplex virus type 1 components necessary to localize transcriptionally active DNA to ND10. J Virol. 2003 May; 77(10):5821-8.
44. Tang Q, Maul GG. Mouse cytomegalovirus immediate-early protein 1 binds with host cell repressors to relieve suppressive effects on viral transcription and replication during lytic infection. J Virol. 2003 Jan; 77(2):1357-67.

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