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Hernan Flores-Rozas

TitleAssociate Professor
Faculty RankAssociate Professor
InstitutionFlorida A&M University
DepartmentCoPPS, IPH Pharmaceutical Sciences
Address1415 S Martin Luther King Jr Blvd
L1-147 East Wing
Tallahassee FL 32307
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    Collapse Biography 
    Collapse awards and honors
    1994Julian R. Rachele Prize, Cornell University
    1996 - 1999Postdoctoral Fellowship, Jane Coffin Childs Memorial Fund
    2002Art Ehrmann Cancer Fund, Fraternal Order of Eagles
    2002 - 2007Distinguished Cancer Scholar, Georgia Cancer Coalition
    2011Minority-Serving Institution Faculty Scholar Award, American Assoc. for Cancer Research
    2015 - 2016Faculty Research Award, FRAP, Florida A&M University
    2020The Scientific Research Honor Society. Full Member., Sigma Xi

    Collapse Overview 
    Collapse overview
    My laboratory investigates the mechanisms by which cells survive cytotoxic stress with the hope of modulating these functions to sensitize cells to chemotherapy and reduce it side-effects. For certain cancer, with no biological therapeutic targets, or as tumors progress, losing them, cytotoxic chemotherapy becomes the only therapeutic alternative. Using a genetic screen in the yeast S. cerevisiae, 71 genes were identified, which when inactivated, hypersensitize cells to anthracyclines, a commonly used chemotherapeutic agent. Mutants defective in genes involved in DNA repair, chromatin remodeling, protein folding and amino acid biosynthesis display the greatest sensitivity to the drug. These genes are potential targets for drugs that will inactivate their function in cancer cell and make them amenable to killing by low doses of chemotherapy and current work is aimed at determining the relevant sites in these proteins which can be used for drug development. The human homologs of these genes have been shown to efficiently complement the yeast defects, protecting the mutants from chemotherapy, suggesting that these genes play similar roles in mammalian cells. We are currently testing this hypothesis by inactivating the human genes in human cancer cells.

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Darling-Reed SF, Nkrumah-Elie Y, Ferguson DT, Flores-Rozas H, Mendonca P, Messeha S, Hudson A, Badisa RB, Tilghman SL, Womble T, Day A, Jett M, Hammamieh R, Soliman KFA. Diallyl Sulfide Attenuation of Carcinogenesis in Mammary Epithelial Cells through the Inhibition of ROS Formation, and DNA Strand Breaks. Biomolecules. 2021 09 06; 11(9). PMID: 34572526.
      Citations: 1     Fields:    Translation:HumansCells
    2. Whitmore A, Freeny D, Sojourner SJ, Miles JS, Graham WM, Flores-Rozas H. Evaluation of the Role of Human DNAJAs in the Response to Cytotoxic Chemotherapeutic Agents in a Yeast Model System. Biomed Res Int. 2020; 2020:9097638. PMID: 32149145.
      Citations: 2     Fields:    Translation:Humans
    3. Miles JS, Sojourner SJ, Whitmore AM, Freeny D, Darling-Reed S, Flores-Rozas H. Synergistic Effect of Endogenous and Exogenous Aldehydes on Doxorubicin Toxicity in Yeast. Biomed Res Int. 2018; 2018:4938189. PMID: 30003101.
      Citations: 2     Fields:    Translation:HumansAnimals
    4. Miles JS, Sojourner SJ, Jaafar L, Whitmore A, Darling-Reed S, Flores-Rozas H. THE ROLE OF PROTEIN CHAPERONES IN THE SURVIVAL FROM ANTHRACYCLINE-INDUCED OXIDATIVE STRESS IN SACCHAROMYCES CEREVISIAE. Int J Adv Res (Indore). 2018 Mar; 6(3):144-152. PMID: 29657945.
    5. Sojourner SJ, Graham WM, Whitmore AM, Miles JS, Freeny D, Flores-Rozas H. The Role of HSP40 Conserved Motifs in the Response to Cytotoxic Stress. J Nat Sci. 2018; 4(4). PMID: 29682607.
    6. Mack N, Mazzio EA, Bauer D, Flores-Rozas H, Soliman KF. Stable shRNA Silencing of Lactate Dehydrogenase A (LDHA) in Human MDA-MB-231 Breast Cancer Cells Fails to Alter Lactic Acid Production, Glycolytic Activity, ATP or Survival. Anticancer Res. 2017 03; 37(3):1205-1212. PMID: 28314283.
      Citations: 21     Fields:    Translation:HumansCells
    7. Nkembo AT, Salako O, Poku RA, Amissah F, Ntantie E, Flores-Rozas H, Lamango NS. Disruption of actin filaments and suppression of pancreatic cancer cell viability and migration following treatment with polyisoprenylated cysteinyl amides. Am J Cancer Res. 2016; 6(11):2532-2546. PMID: 27904769.
    8. Mazu TK, Bricker BA, Flores-Rozas H, Ablordeppey SY. The Mechanistic Targets of Antifungal Agents: An Overview. Mini Rev Med Chem. 2016; 16(7):555-78. PMID: 26776224.
      Citations: 53     Fields:    Translation:HumansAnimals
    9. Taka E, Mazzio EA, Goodman CB, Redmon N, Flores-Rozas H, Reams R, Darling-Reed S, Soliman KF. Anti-inflammatory effects of thymoquinone in activated BV-2 microglial cells. J Neuroimmunol. 2015 Sep 15; 286:5-12. PMID: 26298318.
      Citations: 22     Fields:    Translation:AnimalsCells
    10. Bauer D, Redmon N, Mazzio E, Taka E, Reuben JS, Day A, Sadrud-Din S, Flores-Rozas H, Soliman KF, Darling-Reed S. Diallyl disulfide inhibits TNFa induced CCL2 release through MAPK/ERK and NF-Kappa-B signaling. Cytokine. 2015 Sep; 75(1):117-26. PMID: 26100848.
      Citations: 13     Fields:    Translation:HumansAnimalsCells
    11. Flores-Rozas H, Jaafar L, Xia L. The Role of DNA Mismatch Repair and Recombination in the Processing of DNA Alkylating Damage in Living Yeast Cells. Adv Biosci Biotechnol. 2015; 6(6):408-418. PMID: 26900494.
    12. Freeman MD, Mazu T, Miles JS, Darling-Reed S, Flores-Rozas H. Inactivation of chromatin remodeling factors sensitizes cells to selective cytotoxic stress. Biologics. 2014; 8:269-80. PMID: 25484574.
    13. Stephenson AP, Mazu TK, Miles JS, Freeman MD, Reams RR, Flores-Rozas H. Defects in base excision repair sensitize cells to manganese in S. cerevisiae. Biomed Res Int. 2013; 2013:295635. PMID: 24282812.
      Citations: 1     Fields:    Translation:AnimalsCells
    14. Bolden S, Zhu XY, Etukala JR, Boateng C, Mazu T, Flores-Rozas H, Jacob MR, Khan SI, Walker LA, Ablordeppey SY. Structure-activity relationship (SAR) and preliminary mode of action studies of 3-substituted benzylthioquinolinium iodide as anti-opportunistic infection agents. Eur J Med Chem. 2013; 70:130-42. PMID: 24141203.
      Citations: 4     Fields:    Translation:AnimalsCells
    15. Peng M, Yang Z, Zhang H, Jaafar L, Wang G, Liu M, Flores-Rozas H, Xu J, Mivechi NF, Ko L. GT198 Splice Variants Display Dominant-Negative Activities and Are Induced by Inactivating Mutations. Genes Cancer. 2013 Jan; 4(1-2):26-38. PMID: 23946869.
    16. Banerjee S, Flores-Rozas H. Monoclonal antibodies for targeted therapy in colorectal cancer. Cancer Biol Ther. 2010 Apr 15; 9(8):563-71. PMID: 20200482.
      Citations: 8     Fields:    Translation:HumansAnimals
    17. Labazi M, Jaafar L, Flores-Rozas H. Modulation of the DNA-binding activity of Saccharomyces cerevisiae MSH2-MSH6 complex by the high-mobility group protein NHP6A, in vitro. Nucleic Acids Res. 2009 Dec; 37(22):7581-9. PMID: 19843605.
      Citations: 6     Fields:    Translation:Cells
    18. Jaafar L, Flores-Rozas H. Elucidating the role of human mismatch repair factor hMLH3. Cancer Biol Ther. 2009 Jul; 8(14):1421-3. PMID: 19502813.
      Citations:    Fields:    Translation:HumansAnimalsCells
    19. Xia L, Jaafar L, Cashikar A, Flores-Rozas H. Identification of genes required for protection from doxorubicin by a genome-wide screen in Saccharomyces cerevisiae. Cancer Res. 2007 Dec 01; 67(23):11411-8. PMID: 18056469.
      Citations: 27     Fields:    Translation:Animals
    20. Banerjee S, Flores-Rozas H. Cadmium inhibits mismatch repair by blocking the ATPase activity of the MSH2-MSH6 complex. Nucleic Acids Res. 2005; 33(4):1410-9. PMID: 15746000.
      Citations: 22     Fields:    Translation:AnimalsCells
    21. Lau PJ, Flores-Rozas H, Kolodner RD. Isolation and characterization of new proliferating cell nuclear antigen (POL30) mutator mutants that are defective in DNA mismatch repair. Mol Cell Biol. 2002 Oct; 22(19):6669-80. PMID: 12215524.
      Citations: 40     Fields:    Translation:AnimalsCells
    22. Nakagawa T, Flores-Rozas H, Kolodner RD. The MER3 helicase involved in meiotic crossing over is stimulated by single-stranded DNA-binding proteins and unwinds DNA in the 3' to 5' direction. J Biol Chem. 2001 Aug 24; 276(34):31487-93. PMID: 11376001.
      Citations: 22     Fields:    Translation:Cells
    23. Flores-Rozas H, Clark D, Kolodner RD. Proliferating cell nuclear antigen and Msh2p-Msh6p interact to form an active mispair recognition complex. Nat Genet. 2000 Nov; 26(3):375-8. PMID: 11062484.
      Citations: 101     Fields:    Translation:AnimalsCells
    24. Flores-Rozas H, Kolodner RD. Links between replication, recombination and genome instability in eukaryotes. Trends Biochem Sci. 2000 Apr; 25(4):196-200. PMID: 10754554.
      Citations: 41     Fields:    Translation:Cells
    25. Flores-Rozas H, Kolodner RD. The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations. Proc Natl Acad Sci U S A. 1998 Oct 13; 95(21):12404-9. PMID: 9770499.
      Citations: 132     Fields:    Translation:AnimalsCells
    26. Cai J, Uhlmann F, Gibbs E, Flores-Rozas H, Lee CG, Phillips B, Finkelstein J, Yao N, O'Donnell M, Hurwitz J. Reconstitution of human replication factor C from its five subunits in baculovirus-infected insect cells. Proc Natl Acad Sci U S A. 1996 Nov 12; 93(23):12896-901. PMID: 8917516.
      Citations: 33     Fields:    Translation:HumansAnimalsCells
    27. Uhlmann F, Cai J, Flores-Rozas H, Dean FB, Finkelstein J, O'Donnell M, Hurwitz J. In vitro reconstitution of human replication factor C from its five subunits. Proc Natl Acad Sci U S A. 1996 Jun 25; 93(13):6521-6. PMID: 8692848.
      Citations: 17     Fields:    Translation:HumansCells
    28. Valdez BC, Henning D, Busch RK, Woods K, Flores-Rozas H, Hurwitz J, Perlaky L, Busch H. A nucleolar RNA helicase recognized by autoimmune antibodies from a patient with watermelon stomach disease. Nucleic Acids Res. 1996 Apr 01; 24(7):1220-4. PMID: 8614622.
      Citations: 27     Fields:    Translation:HumansCells
    29. Pan ZQ, Reardon JT, Li L, Flores-Rozas H, Legerski R, Sancar A, Hurwitz J. Inhibition of nucleotide excision repair by the cyclin-dependent kinase inhibitor p21. J Biol Chem. 1995 Sep 15; 270(37):22008-16. PMID: 7665622.
      Citations: 44     Fields:    Translation:HumansCells
    30. Flores-Rozas H, Kelman Z, Dean FB, Pan ZQ, Harper JW, Elledge SJ, O'Donnell M, Hurwitz J. Cdk-interacting protein 1 directly binds with proliferating cell nuclear antigen and inhibits DNA replication catalyzed by the DNA polymerase delta holoenzyme. Proc Natl Acad Sci U S A. 1994 Aug 30; 91(18):8655-9. PMID: 7915843.
      Citations: 109     Fields:    Translation:HumansCells
    31. Flores-Rozas H, Hurwitz J. Characterization of a new RNA helicase from nuclear extracts of HeLa cells which translocates in the 5' to 3' direction. J Biol Chem. 1993 Oct 05; 268(28):21372-83. PMID: 8407977.
      Citations: 23     Fields:    Translation:HumansCells
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