Comet Assay

"Comet Assay" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure, which enables searching at various levels of specificity.

MeSH information

Definition | Details | More General Concepts | Related Concepts | More Specific Concepts

A genotoxicological technique for measuring DNA damage in an individual cell using single-cell gel electrophoresis. Cell DNA fragments assume a "comet with tail" formation on electrophoresis and are detected with an image analysis system. Alkaline assay conditions facilitate sensitive detection of single-strand damage.

Descriptor ID	D020552
MeSH Number(s)	E05.196.401.153.150 E05.301.300.100.150 E05.393.560.150 E05.940.560.150
Concept/Terms	Comet Assay Comet Assay Assay, Comet Assays, Comet Comet Assays Gel Electrophoresis, Single-Cell Electrophoreses, Single-Cell Gel Electrophoresis, Single-Cell Gel Gel Electrophoreses, Single-Cell Gel Electrophoresis, Single Cell Single-Cell Gel Electrophoreses Single-Cell Gel Electrophoresis Electrophoresis, Gel, Single-Cell Alkaline Comet Assay Alkaline Comet Assay Alkaline Comet Assays Assay, Alkaline Comet Assays, Alkaline Comet Comet Assay, Alkaline Comet Assays, Alkaline Alkaline Single-Cell Gel Electrophoresis Assay Alkaline Single Cell Gel Electrophoresis Assay

Below are MeSH descriptors whose meaning is more general than "Comet Assay".

Analytical, Diagnostic and Therapeutic Techniques and Equipment [E]
Investigative Techniques [E05]
Chemistry Techniques, Analytical [E05.196]
Electrophoresis [E05.196.401]
Electrophoresis, Agar Gel [E05.196.401.153]
Comet Assay [E05.196.401.153.150]
Electrochemical Techniques [E05.301]
Electrophoresis [E05.301.300]
Electrophoresis, Agar Gel [E05.301.300.100]
Comet Assay [E05.301.300.100.150]
Genetic Techniques [E05.393]
Mutagenicity Tests [E05.393.560]
Comet Assay [E05.393.560.150]
Toxicity Tests [E05.940]
Mutagenicity Tests [E05.940.560]
Comet Assay [E05.940.560.150]

Below are MeSH descriptors whose meaning is related to "Comet Assay".

Below are MeSH descriptors whose meaning is more specific than "Comet Assay".

publications

Timeline | Most Recent

This graph shows the total number of publications written about "Comet Assay" by people in this website by year, and whether "Comet Assay" was a major or minor topic of these publications.

Bar chart showing 45 publications over 18 distinct years, with a maximum of 6 publications in 2010

To see the data from this visualization as text, click here.

Year	Major Topic	Minor Topic	Total
2001	0	1	1
2003	0	1	1
2004	0	4	4
2005	0	2	2
2006	0	2	2
2007	1	2	3
2008	0	3	3
2009	0	2	2
2010	0	6	6
2011	0	3	3
2012	1	3	4
2013	1	1	2
2014	0	2	2
2015	0	4	4
2016	0	1	1
2018	0	1	1
2019	2	1	3
2020	0	1	1

Below are the most recent publications written about "Comet Assay" by people in Profiles.

M?ller P, Azqueta A, Boutet-Robinet E, Koppen G, Bonassi S, Milic M, Gajski G, Costa S, Teixeira JP, Costa Pereira C, Dusinska M, Godschalk R, Brunborg G, Gutzkow KB, Giovannelli L, Cooke MS, Richling E, Laffon B, Valdiglesias V, Basaran N, Del Bo' C, Zegura B, Novak M, Stopper H, Vodicka P, Vodenkova S, de Andrade VM, Sramkova M, Gabelova A, Collins A, Langie SAS. Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. Nat Protoc. 2020 12; 15(12):3817-3826.

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Mbemi AT, Sims JN, Yedjou CG, Noubissi FK, Gomez CR, Tchounwou PB. Vernonia calvoana Shows Promise towards the Treatment of Ovarian Cancer. Int J Mol Sci. 2020 Jun 22; 21(12).

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Karbaschi M, Ji Y, Abdulwahed AMS, Alohaly A, Bedoya JF, Burke SL, Boulos TM, Tempest HG, Cooke MS. Evaluation of the Major Steps in the Conventional Protocol for the Alkaline Comet Assay. Int J Mol Sci. 2019 Dec 02; 20(23).

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Sizochenko N, Syzochenko M, Fjodorova N, Rasulev B, Leszczynski J. Evaluating genotoxicity of metal oxide nanoparticles: Application of advanced supervised and unsupervised machine learning techniques. Ecotoxicol Environ Saf. 2019 Dec 15; 185:109733.

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Ji Y, Karbaschi M, Cooke MS. Mycoplasma infection of cultured cells induces oxidative stress and attenuates cellular base excision repair activity. Mutat Res Genet Toxicol Environ Mutagen. 2019 Sep; 845:403054.

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Nautiyal A, Mondal T, Mukherjee A, Mitra D, Kaushik A, Goel HC, Goel A, Dey SK. Quantification of DNA damage in patients undergoing non-contrast and contrast enhanced whole body PET/CT investigations using comet assay and micronucleus assay. Int J Radiat Biol. 2019 06; 95(6):710-719.

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Golbamaki A, Golbamaki N, Sizochenko N, Rasulev B, Leszczynski J, Benfenati E. Genotoxicity induced by metal oxide nanoparticles: a weight of evidence study and effect of particle surface and electronic properties. Nanotoxicology. 2018 12; 12(10):1113-1129.

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Kim G, Karbaschi M, Cooke M, Gaitas A. Light-based methods for whole blood bacterial inactivation enabled by a recirculating flow system. Photochem Photobiol. 2018 07; 94(4):744-751.

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Skipper A, Sims JN, Yedjou CG, Tchounwou PB. Cadmium Chloride Induces DNA Damage and Apoptosis of Human Liver Carcinoma Cells via Oxidative Stress. Int J Environ Res Public Health. 2016 Jan 02; 13(1).

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Yedjou CG, Tchounwou HM, Tchounwou PB. DNA Damage, Cell Cycle Arrest, and Apoptosis Induction Caused by Lead in Human Leukemia Cells. Int J Environ Res Public Health. 2015 Dec 22; 13(1):ijerph13010056.

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