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Linda Spatz
Title Associate Professor, Department of Microbiology
Faculty Rank
Degree Ph.D.
Institution City College, CUNY
Department Department of Pathobiology at Sophie Davis
Clusters Infectious and Immunological Diseases
Address
City College, CUNY, Harris Hall Rm 210 C
160 Convent Avenue
City New York
State NY
Postal Code 10031
Telephone 212-650-77-3
Fax 212-650-7797
Email
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Awards and Honors icon

2014 CNY President''s award for outstanding faculty service

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  Dr. Linda Spatz is interested in understanding how anti-double stranded (anti-dsDNA) antibodies which are the hallmark of the autoimmune disease Systemic Lupus Erythematosus (SLE), arise. Her laboratory is studying the regulation of anti-dsDNA B-cells. Normally these B cells are kept from secreting transgenic anti-ds DNA antibodies by regulatory mechanisms, collectively known as B cell tolerance. When one or more of these regulatory mechanisms goes awry, a breakdown in tolerance ensues and anti-dsDNA antibodies are secreted at elevated levels. These antibodies can deposit in various organs of the body including the skin, joints, and kidney where they can induce tissue damage. Dr. Spatz's laboratory is investigating potential genetic and environmental factors that can lead to a breakdown in anti-dsDNA B cell tolerance. Using an anti-dsDNA transgenic mouse model she is currently studying how the overexpression of a B cell survival factor known as BAFF, can lead to the loss of B cell tolerance and the secretion of transgenic anti-dsDNA antibodies.

In addition, Dr. Spatz is working in collaboration with Dr. Paul Gottlieb to study the role of the Epstein Barr virus (EBV) in the etiology of SLE. They have observed that immunization of mice with a major nuclear protein of EBV known as EBNA-1 can elicit the production of pathogenic anti-dsDNA antibodies that can deposit in the kidney. Future studies are addressing the mechanism by which EBNA-1 can elicit these anti-dsDNA antibodies.

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1. Yadav P, Carr MT, Yu R, Mumbey-Wafula A, Spatz LA. Mapping an epitope in EBNA-1 that is recognized by monoclonal antibodies to EBNA-1 that cross-react with dsDNA. Immun Inflamm Dis. 2016 Sep; 4(3):362-75.
2. Alimova A, Wei H, Katz A, Spatz L, Gottlieb P. The ?6 cystovirus protein P7 becomes accessible to antibodies in the transcribing nucleocapsid: a probe for viral structural elements. PLoS One. 2015; 10(3):e0122160.
3. Klein F, Halper-Stromberg A, Horwitz JA, Gruell H, Scheid JF, Bournazos S, Mouquet H, Spatz LA, Diskin R, Abadir A, Zang T, Dorner M, Billerbeck E, Labitt RN, Gaebler C, Marcovecchio P, Incesu RB, Eisenreich TR, Bieniasz PD, Seaman MS, Bjorkman PJ, Ravetch JV, Ploss A, Nussenzweig MC. HIV therapy by a combination of broadly neutralizing antibodies in humanized mice. Nature. 2012 Dec 06; 492(7427):118-22.
4. Yadav P, Tran H, Ebegbe R, Gottlieb P, Wei H, Lewis RH, Mumbey-Wafula A, Kaplan A, Kholdarova E, Spatz L. Antibodies elicited in response to EBNA-1 may cross-react with dsDNA. PLoS One. 2011 Jan 04; 6(1):e14488.
5. Crupi R, Cambiaghi M, Spatz L, Hen R, Thorn M, Friedman E, Vita G, Battaglia F. Reduced adult neurogenesis and altered emotional behaviors in autoimmune-prone B-cell activating factor transgenic mice. Biol Psychiatry. 2010 Mar 15; 67(6):558-66.
6. Thorn M, Lewis RH, Mumbey-Wafula A, Kantrowitz S, Spatz LA. BAFF overexpression promotes anti-dsDNA B-cell maturation and antibody secretion. Cell Immunol. 2010; 261(1):9-22.
7. Taylor DK, Ito E, Thorn M, Sundar K, Tedder T, Spatz LA. Loss of tolerance of anti-dsDNA B cells in mice overexpressing CD19. Mol Immunol. 2006 Apr; 43(11):1776-90.
8. Sundar K, Jacques S, Gottlieb P, Villars R, Benito ME, Taylor DK, Spatz LA. Expression of the Epstein-Barr virus nuclear antigen-1 (EBNA-1) in the mouse can elicit the production of anti-dsDNA and anti-Sm antibodies. J Autoimmun. 2004 Sep; 23(2):127-40.
9. Chu YP, Spatz L, Diamond B. A second heavy chain permits survival of high affinity autoreactive B cells. Autoimmunity. 2004 Feb; 37(1):27-32.
10. Chu YP, Taylor D, Yan HG, Diamond B, Spatz L. Persistence of partially functional double-stranded (ds) DNA binding B cells in mice transgenic for the IgM heavy chain of an anti-dsDNA antibody. Int Immunol. 2002 Jan; 14(1):45-54.
11. Peeva E, Grimaldi C, Spatz L, Diamond B. Bromocriptine restores tolerance in estrogen-treated mice. J Clin Invest. 2000 Dec; 106(11):1373-9.
12. Bynoe MS, Spatz L, Diamond B. Characterization of anti-DNA B cells that escape negative selection. Eur J Immunol. 1999 04; 29(4):1304-13.
13. Spatz L, Saenko V, Iliev A, Jones L, Geskin L, Diamond B. Light chain usage in anti-double-stranded DNA B cell subsets: role in cell fate determination. J Exp Med. 1997 Apr 07; 185(7):1317-26.
14. Gaynor B, Putterman C, Valadon P, Spatz L, Scharff MD, Diamond B. Peptide inhibition of glomerular deposition of an anti-DNA antibody. Proc Natl Acad Sci U S A. 1997 Mar 04; 94(5):1955-60.
15. Spatz L, Iliev A, Saenko V, Jones L, Irigoyen M, Manheimer-Lory A, Gaynor B, Putterman C, Bynoe M, Kowal C, Kuo P, Newman J, Diamond B. Studies on the structure, regulation, and pathogenic potential of anti-dsDNA antibodies. Methods. 1997 Jan; 11(1):70-8.
16. Iliev A, Spatz L, Ray S, Diamond B. Lack of allelic exclusion permits autoreactive B cells to escape deletion. J Immunol. 1994 Oct 15; 153(8):3551-6.
17. Offen D, Spatz L, Escowitz H, Factor S, Diamond B. Induction of tolerance to an IgG autoantibody. Proc Natl Acad Sci U S A. 1992 Sep 01; 89(17):8332-6.
18. Spatz LA, Daugherty BL, DeMartino JA, Mark GE, Latov N. Expression of recombinant human anti-MAG antibodies in non-lymphoid mammalian cells. Hum Antibodies Hybridomas. 1992 Apr; 3(2):107-11.
19. Spatz LA, Williams M, Brender B, Desai R, Latov N. DNA sequence analysis and comparison of the variable heavy and light chain regions of two IgM, monoclonal, anti-myelin associated glycoprotein antibodies. J Neuroimmunol. 1992 Jan; 36(1):29-39.
20. Lee KW, Inghirami G, Spatz L, Knowles DM, Latov N. The B-cells that express anti-MAG antibodies in neuropathy and non-malignant IgM monoclonal gammopathy belong to the CD5 subpopulation. J Neuroimmunol. 1991 Jan; 31(1):83-8.
21. Desai R, Spatz L, Matsuda T, Ilyas AA, Berman JE, Alt FW, Kabat EA, Latov N. Molecular cloning of a human immunoglobulin heavy chain variable (vh) region with anti-myelin-associated glycoprotein activity. J Neuroimmunol. 1990 Dec; 30(2-3):245.
22. Spatz LA, Wong KK, Williams M, Desai R, Golier J, Berman JE, Alt FW, Latov N. Cloning and sequence analysis of the VH and VL regions of an anti-myelin/DNA antibody from a patient with peripheral neuropathy and chronic lymphocytic leukemia. J Immunol. 1990 Apr 01; 144(7):2821-8.
23. Desai R, Spatz L, Matsuda T, Ilyas AA, Berman JE, Alt FW, Kabat EA, Latov N. Molecular cloning of a human immunoglobulin heavy chain variable (VH) region with anti-myelin-associated glycoprotein activity. J Neuroimmunol. 1990 Jan; 26(1):35-41.
24. Spatz L, Lieberson B, Abraham J, Miller OJ, Miller DA, Latov N. Generation of human B-cell hybridomas secreting monoclonal anti-myelin-associated glycoprotein antibodies from a patient with neuropathy. Ann Neurol. 1987 Feb; 21(2):207-11.
25. Freddo L, Hays AP, Nickerson KG, Spatz L, McGinnis S, Lieberson R, Vedeler CA, Shy ME, Autilio-Gambetti L, Grauss FC, et al. Monoclonal anti-DNA IgM kappa in neuropathy binds to myelin and to a conformational epitope formed by phosphatidic acid and gangliosides. J Immunol. 1986 Dec 15; 137(12):3821-5.
26. Spatz L, Latov N. Secretions of anti-myelin-associated glycoprotein antibodies by B cells from patients with neuropathy and nonmalignant monoclonal gammopathy. Cell Immunol. 1986 Dec; 103(2):434-40.
27. Friedman E, Nilaver G, Carmel P, Perlow M, Spatz L, Latov N. Myelination by transplanted fetal and neonatal oligodendrocytes in a dysmyelinating mutant. Brain Res. 1986 Jul 16; 378(1):142-6.
28. Latov N, Godfrey M, Thomas Y, Nobile-Orazio E, Spatz L, Abraham J, Perman G, Freddo L, Chess L. Neuropathy and anti-myelin-associated glycoprotein IgM M proteins: T cell regulation of M protein secretion in vitro. Ann Neurol. 1985 Aug; 18(2):182-8.

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1. Spatz, L. Tolerance and Autoimmunity (chapter). Immunology, A Short Course. 2009.

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1. Klein F, Halper-Stromberg A, Horwitz JA, Gruell H, Scheid JF, Bournazos S, Mouquet H, Spatz LA, Diskin R, Abadir A, Zang T, Dorner M, Billerbeck E, Labitt RN, Gaebler C, Marcovecchio PM, Incesu RB, Eisenreich TR, Bieniasz PD, Seaman MS, Bjorkman PJ, Ravetch JV, Ploss A, Nussenzweig MC. . Nature. HIV therapy by a combination of broadly neutralizing antibodies in humanized mice. 2012; 492:118-122.
2. Latov, N., M. Godfrey, Y. Thomas, E. Nobile-Orazio, L. Spatz, J. Abraham, G. Perman, L. Freddo, and L. Chess. Ann. Neurol. Regulation of anti-MAG M-protein secretion in a patient with neuropathy and macroglobulinemia: T and B cell interactions in vitro. 1985; 18:182-188.
3. Spatz, L., L. Whitman, M.-J. Messito, G. Nilaver, S. Ginsberg, and N. Latov. Immunol. Comm. Measurement of myelin basic protein antibodies by ELISA utilizing biotinylated antibodies. 1983; 12:31-37.

Reviews/Chapters/Editorials icon

 

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1. Khalil, M., L. Spatz, and B. Diamond. Systemic Lupus Erythematosus (R. G. Lahita, Ed.), Academic Press, San Diego. Amto-DNA antibodies. 1998.
2. Hohmann, A. W., L. Spatz, M. Irigoyen, and A. Manheimer-Lory. Monoclonal Antibodies, The Second Generation. Human monoclonal antibodies from immortalized B cells. 1994; 43-66.

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DNA
Antibodies, Antinuclear
B-Lymphocytes
Epstein-Barr Virus Nuclear Antigens
Mice
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