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Suranganie Dharmawardhane
Title Dr.
Faculty Rank Associate Professor
Degree Ph.D.
Institution University of Puerto Rico
Department Biochemistry
Clusters Cancer
Address
UPR-Medical Sciences Campus
School of Medicine
Department of Biochemistry, 6th Floor, GAB
P.O. Box 365067
City San Juan
State PR
Postal Code 00936-5067
Telephone (787)758-2525 ext. 1630
Fax
Email
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Other Positions icon

Title Adjunct Associate Professor
Institution Universidad Central del Caribe
Department Biochemistry
Division "Cancer","Cancer"

Awards and Honors icon

1999 American Heart Association, National Scientist Development Award

Narrative icon

  I have spent the past 13 years directing a viable program in breast cancer metastasis and the preventive and therapeutic role of natural and synthetic anti-metastasis compounds. My postdoctoral training was from Dr. John Condeelis (Albert Einstein College of Medicine, NY), who is a pioneering authority on in vivo image analysis of breast cancer metastasis. I was subsequently trained in molecular biology and analysis of cell signaling of model systems (Dictyostelium) and fibrobalsts and leukocytes in the laboratories of Dr. Richard Firtel (University of California, San Diego) and Dr. Gary Bokoch (Scripps Research Institute, CA). During my Assistant Professorship at the University of Texas at Austin (1999-2005), in collaboration with biomedical engineers, we designed and developed methodology for whole body and microscopic fluorescence image analysis of fluorescently-tagged breast cancer cells in mouse models. This technology and my research program in breast cancer metastasis have been transferred to my current research group at the University of Puerto Rico Medical Sciences Campus (UPR MSC), San Juan, PR.

At UPR MSC, we have continued with investigation of the effects and molecular mechanisms of red wine and soy polyphenols using human breast cancer cell lines and immunocompromised mouse models. In collaboration with medicinal chemists (Drs. Eliud Hernandez and Cornelis Vlaar, School of Pharmacy), we are designing and characterizing novel small molecule inhibitors to the small GTPase Rac, a central regulator of motility in normal and cancer cells. We have a pending patent for the small molecule EHop-016 that we are developing as an anti metastatic breast cancer therapeutic. Our recent collaborations with Dr. George Calin (MD Anderson Cancer Center, Houston, TX), has led to an investigation on the role of non-coding RNA (ncRNA) in breast cancer therapy resistance. We have also initiated a collaborative study with Dr. Naima Moustaid-Moussa (Texas Tech University) to investigate molecular mechanisms and targeted therapeutics for obesity-associated breast cancer metastasis in postmenopausal women.

My commitment to cancer research is reflected in 39 pubmed citations, of which 18 (2002-2012) are from my independent research program in breast cancer. I am an active member of the American Society of Cell Biologists (ASCB), American Association for Cancer Research (AACR), and the American Society for Biochemistry and Molecular Biology (ASBMB). I have also served as a review panel member of the US Army Department of Defense (DoD)/Ovarian Cancer Research and Breast Cancer Research Programs (BCRP) and acted as an ad-hoc reviewer for the NIH Basic Mechanisms of Cancer Therapeutics study section. My research has received funding from NIH/NCI, DoD/BCRP and the American Institute of Cancer Research. I was also the recipient of a Cancer Research Center Grant (role: Director) that I submitted with the RCMI Program of Universidad Central del Caribe (UCC), 5G12RR003035, that was relinquished after my move to UPR MSC.

I also enjoy and have had successful experiences with mentoring undergraduate, graduate, medical, and post-doctoral students, as well as Assistant Professors. Most of my undergraduate mentees have gone on to Ph.D and M.D. Programs. I graduated two M.Sc. students and 5 Ph.D. students who went on to postdoctoral positions in prestigious institutions. My first graduate student Dr. Paige Baugher is a tenured Associate Professor with her own breast cancer research program at Pacific University, Oregon. I have trained eight postdoctoral (6 Ph.D.s and 2 M.D.s) researchers who are currently in academia or medical residencies. During my tenure at UCC (Bayamon, PR) as chief of the Cell Biology section, I mentored two assistant professors: Dr. Valance Washington was the successful recipient of a MBRS/SC2, who then went on to receive a R01 award. Dr. Michelle Martínez currently holds three pilot project awards from NIH grants to UCC and UPR. My current mentee from the UPRCCC, Dr. Pablo Vivas was recently awarded a NIH/NCI/K22 grant. I am currently training four Ph.D. students and an Assistant Professor in my research group. Therefore, my extensive training record exemplifies my commitment to mentoring women and minorities to become productive biomedical researchers and academics.

NIH Awarded Grants icon

  Research projects funded by the National Institutes of Health (NIH), the Centers for Disease Control (CDC), the Food and Drug Administration (FDA), and the Department of Veterans Affairs (VA)

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1.
2017Developmental Research Project Program5P20GM103475-16
2.
2017Molecular targets of soy isoflavones in breast cancer progression5SC3GM084824-08
3.
2016Molecular targets of soy isoflavones in breast cancer progression5SC3GM084824-07
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2016Developmental Research Project Program5P20GM103475-15
5.
2015Molecular targets of soy isoflavones in breast cancer progression5SC3GM084824-06
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2015Developmental Research Project Program2P20GM103475-14
7.
2014Molecular targets of soy isoflavones in breast cancer progression2SC3GM084824-05
8.
2011Molecular targets of soy isoflavones in breast cancer progression5SC3GM084824-04
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2010Molecular targets of soy isoflavones in breast cancer progression3SC3GM084824-02S1
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2010CANCER RESEARCH CENTER5G12RR003035-25
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2010Molecular targets of soy isoflavones in breast cancer progression5SC3GM084824-03
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2009Molecular targets of soy isoflavones in breast cancer progression7SC3GM084824-02
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2008Molecular targets of soy isoflavones in breast cancer progression1SC3GM084824-01
14.
2006Resveratrol in breast cancer cell survival or apoptosis5R03CA109913-03
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2005Resveratrol in breast cancer cell survival or apoptosis7R03CA109913-02
16.
2001REGULATORY ROLE OF RAC AND CDC42 ON TUMOR METASTASIS5R21CA083957-02
17.
2000REGULATORY ROLE OF RAC AND CDC42 ON TUMOR METASTASIS1R21CA083957-01A1

Publications icon

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1. Al-Jawadi A, Moussa H, Ramalingam L, Dharmawardhane S, Gollahon L, Gunaratne P, Layeequr Rahman R, Moustaid-Moussa N. Protective properties of n-3 fatty acids and implications in obesity-associated breast cancer. J Nutr Biochem. 2018 Mar; 53:1-8.
2. Humphries-Bickley T, Castillo-Pichardo L, Hernandez-O'Farrill E, Borrero-Garcia LD, Forestier-Roman I, Gerena Y, Blanco M, Rivera-Robles MJ, Rodriguez-Medina JR, Cubano LA, Vlaar CP, Dharmawardhane S. Characterization of a Dual Rac/Cdc42 Inhibitor MBQ-167 in Metastatic Cancer. Mol Cancer Ther. 2017 May; 16(5):805-818.
3. Rivera Rivera A, Castillo-Pichardo L, Gerena Y, Dharmawardhane S. Anti-Breast Cancer Potential of Quercetin via the Akt/AMPK/Mammalian Target of Rapamycin (mTOR) Signaling Cascade. PLoS One. 2016; 11(6):e0157251.
4. de la Parra C, Castillo-Pichardo L, Cruz-Collazo A, Cubano L, Redis R, Calin GA, Dharmawardhane S. Soy Isoflavone Genistein-Mediated Downregulation of miR-155 Contributes to the Anticancer Effects of Genistein. Nutr Cancer. 2016; 68(1):154-64.
5. de la Parra C, Borrero-Garcia LD, Cruz-Collazo A, Schneider RJ, Dharmawardhane S. Equol, an isoflavone metabolite, regulates cancer cell viability and protein synthesis initiation via c-Myc and eIF4G. J Biol Chem. 2015 Mar 06; 290(10):6047-57.
6. Humphries-Bickley T, Castillo-Pichardo L, Corujo-Carro F, Duconge J, Hernandez-O'Farrill E, Vlaar C, Rodriguez-Orengo JF, Cubano L, Dharmawardhane S. Pharmacokinetics of Rac inhibitor EHop-016 in mice by ultra-performance liquid chromatography tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Feb 15; 981-982:19-26.
7. Cekanova M, Fernando RI, Siriwardhana N, Sukhthankar M, De la Parra C, Woraratphoka J, Malone C, Ström A, Baek SJ, Wade PA, Saxton AM, Donnell RM, Pestell RG, Dharmawardhane S, Wimalasena J. BCL-2 family protein, BAD is down-regulated in breast cancer and inhibits cell invasion. Exp Cell Res. 2015 Feb 01; 331(1):1-10.
8. Castillo-Pichardo L, Dharmawardhane S, Rodríguez-Orengo JF. Rapid quantification of resveratrol in mouse plasma by ultra high pressure liquid chromatography (UPLC) coupled to tandem mass spectrometry. P R Health Sci J. 2014 Dec; 33(4):151-6.
9. Castillo-Pichardo L, Humphries-Bickley T, De La Parra C, Forestier-Roman I, Martinez-Ferrer M, Hernandez E, Vlaar C, Ferrer-Acosta Y, Washington AV, Cubano LA, Rodriguez-Orengo J, Dharmawardhane S. The Rac Inhibitor EHop-016 Inhibits Mammary Tumor Growth and Metastasis in a Nude Mouse Model. Transl Oncol. 2014 Oct; 7(5):546-55.
10. Ramos G, Loperena Y, Ortiz G, Reyes F, Szeto A, Vera J, Velez J, Morales J, Morrero D, Castillo L, Dharmawardhane S, Melendez E, Washington AV. The addition of a pregnenolone pendant group enhances the anticancer properties of titanocene dichloride in a mcf-7 xenograft model. Anticancer Res. 2014 Apr; 34(4):1609-15.
11. Martin H, Mali RS, Ma P, Chatterjee A, Ramdas B, Sims E, Munugalavadla V, Ghosh J, Mattingly RR, Visconte V, Tiu RV, Vlaar CP, Dharmawardhane S, Kapur R. Pak and Rac GTPases promote oncogenic KIT-induced neoplasms. J Clin Invest. 2013 Oct; 123(10):4449-63.
12. Dharmawardhane S, Hernandez E, Vlaar C. Development of EHop-016: a small molecule inhibitor of Rac. Enzymes. 2013; 33 Pt A:117-46.
13. Castillo-Pichardo L, Cubano LA, Dharmawardhane S. Dietary grape polyphenol resveratrol increases mammary tumor growth and metastasis in immunocompromised mice. BMC Complement Altern Med. 2013 Jan 08; 13:6.
14. de la Parra C, Otero-Franqui E, Martinez-Montemayor M, Dharmawardhane S. The soy isoflavone equol may increase cancer malignancy via up-regulation of eukaryotic protein synthesis initiation factor eIF4G. J Biol Chem. 2012 Dec 07; 287(50):41640-50.
15. Montalvo-Ortiz BL, Castillo-Pichardo L, Hernández E, Humphries-Bickley T, De la Mota-Peynado A, Cubano LA, Vlaar CP, Dharmawardhane S. Characterization of EHop-016, novel small molecule inhibitor of Rac GTPase. J Biol Chem. 2012 Apr 13; 287(16):13228-38.
16. Castillo-Pichardo L, Dharmawardhane SF. Grape polyphenols inhibit Akt/mammalian target of rapamycin signaling and potentiate the effects of gefitinib in breast cancer. Nutr Cancer. 2012; 64(7):1058-69.
17. Martínez-Montemayor MM, Acevedo RR, Otero-Franqui E, Cubano LA, Dharmawardhane SF. Ganoderma lucidum (Reishi) inhibits cancer cell growth and expression of key molecules in inflammatory breast cancer. Nutr Cancer. 2011; 63(7):1085-94.
18. Hernández E, De La Mota-Peynado A, Dharmawardhane S, Vlaar CP. Novel inhibitors of Rac1 in metastatic breast cancer. P R Health Sci J. 2010 Dec; 29(4):348-56.
19. Martínez-Montemayor MM, Otero-Franqui E, Martinez J, De La Mota-Peynado A, Cubano LA, Dharmawardhane S. Individual and combined soy isoflavones exert differential effects on metastatic cancer progression. Clin Exp Metastasis. 2010 Oct; 27(7):465-80.
20. Castillo-Pichardo L, Martínez-Montemayor MM, Martínez JE, Wall KM, Cubano LA, Dharmawardhane S. Inhibition of mammary tumor growth and metastases to bone and liver by dietary grape polyphenols. Clin Exp Metastasis. 2009; 26(6):505-16.
21. Schlachterman A, Valle F, Wall KM, Azios NG, Castillo L, Morell L, Washington AV, Cubano LA, Dharmawardhane SF. Combined resveratrol, quercetin, and catechin treatment reduces breast tumor growth in a nude mouse model. Transl Oncol. 2008 Mar; 1(1):19-27.
22. Calabria LM, Piacente S, Kapusta I, Dharmawardhane SF, Segarra FM, Pessiki PJ, Mabry TJ. Triterpene saponins from Silphium radula. Phytochemistry. 2008 Feb; 69(4):961-72.
23. Azios NG, Krishnamoorthy L, Harris M, Cubano LA, Cammer M, Dharmawardhane SF. Estrogen and resveratrol regulate Rac and Cdc42 signaling to the actin cytoskeleton of metastatic breast cancer cells. Neoplasia. 2007 Feb; 9(2):147-58.
24. Sokolov K, Nida D, Descour M, Lacy A, Levy M, Hall B, Dharmawardhane S, Ellington A, Korgel B, Richards-Kortum R. Molecular optical imaging of therapeutic targets of cancer. Adv Cancer Res. 2007; 96:299-344.
25. Carlson AL, Hoffmeyer MR, Wall KM, Baugher PJ, Richards-Kortum R, Dharmawardhane SF. In situ analysis of breast cancer progression in murine models using a macroscopic fluorescence imaging system. Lasers Surg Med. 2006 Dec; 38(10):928-38.
26. Baugher PJ, Krishnamoorthy L, Price JE, Dharmawardhane SF. Rac1 and Rac3 isoform activation is involved in the invasive and metastatic phenotype of human breast cancer cells. Breast Cancer Res. 2005; 7(6):R965-74.
27. Hoffmeyer MR, Wall KM, Dharmawardhane SF. In vitro analysis of the invasive phenotype of SUM 149, an inflammatory breast cancer cell line. Cancer Cell Int. 2005 Apr 27; 5(1):11.
28. Azios NG, Dharmawardhane SF. Resveratrol and estradiol exert disparate effects on cell migration, cell surface actin structures, and focal adhesion assembly in MDA-MB-231 human breast cancer cells. Neoplasia. 2005 Feb; 7(2):128-40.
29. Hsu ER, Anslyn EV, Dharmawardhane S, Alizadeh-Naderi R, Aaron JS, Sokolov KV, El-Naggar AK, Gillenwater AM, Richards-Kortum RR. A far-red fluorescent contrast agent to image epidermal growth factor receptor expression. Photochem Photobiol. 2004 Mar; 79(3):272-9.
30. Brownson DM, Azios NG, Fuqua BK, Dharmawardhane SF, Mabry TJ. Flavonoid effects relevant to cancer. J Nutr. 2002 Nov; 132(11 Suppl):3482S-3489S.
31. Lacy AA, Collier T, Price JE, Dharmawardhane S, Richards-Kortum R. Near real-time in vivo confocal imaging of mouse mammary tumors. Front Biosci. 2002 Jan 01; 7:f1-7.
32. Dharmawardhane S, Schürmann A, Sells MA, Chernoff J, Schmid SL, Bokoch GM. Regulation of macropinocytosis by p21-activated kinase-1. Mol Biol Cell. 2000 Oct; 11(10):3341-52.
33. Dharmawardhane S, Brownson D, Lennartz M, Bokoch GM. Localization of p21-activated kinase 1 (PAK1) to pseudopodia, membrane ruffles, and phagocytic cups in activated human neutrophils. J Leukoc Biol. 1999 Sep; 66(3):521-7.
34. Dharmawardhane S, Sanders LC, Martin SS, Daniels RH, Bokoch GM. Localization of p21-activated kinase 1 (PAK1) to pinocytic vesicles and cortical actin structures in stimulated cells. J Cell Biol. 1997 Sep 22; 138(6):1265-78.
35. Rietdorf J, Siegert F, Dharmawardhane S, Firtel RA, Weijer CJ. Analysis of cell movement and signalling during ring formation in an activated G alpha1 mutant of Dictyostelium discoideum that is defective in prestalk zone formation. Dev Biol. 1997 Jan 01; 181(1):79-90.
36. Dharmawardhane S, Bokoch GM. Rho GTPases and leukocyte cytoskeletal regulation. Curr Opin Hematol. 1997 Jan; 4(1):12-8.
37. Dharmawardhane S, Cubitt AB, Clark AM, Firtel RA. Regulatory role of the G alpha 1 subunit in controlling cellular morphogenesis in Dictyostelium. Development. 1994 Dec; 120(12):3549-61.
38. Carrel F, Dharmawardhane S, Clark AM, Powell-Coffman JA, Firtel RA. Spatial and temporal expression of the Dictyostelium discoideum G alpha protein subunit G alpha 2: expression of a dominant negative protein inhibits proper prestalk to stalk differentiation. Mol Biol Cell. 1994 Jan; 5(1):7-16.
39. Schatzle J, Bush J, Dharmawardhane S, Firtel RA, Gomer RH, Cardelli J. Characterization of the signal transduction pathways and cis-acting DNA sequence responsible for the transcriptional induction during growth and development of the lysosomal alpha-mannosidase gene in Dictyostelium discoideum. J Biol Chem. 1993 Sep 15; 268(26):19632-9.
40. Cubitt AB, Dharmawardhane S, Firtel RA. Developmentally regulated changes in 1,2-diacylglycerol in Dictyostelium. Regulation by light and G proteins. J Biol Chem. 1993 Aug 15; 268(23):17431-9.
41. Cubitt AB, Carrel F, Dharmawardhane S, Gaskins C, Hadwiger J, Howard P, Mann SK, Okaichi K, Zhou K, Firtel RA. Molecular genetic analysis of signal transduction pathways controlling multicellular development in Dictyostelium. Cold Spring Harb Symp Quant Biol. 1992; 57:177-92.
42. Dharmawardhane S, Demma M, Yang F, Condeelis J. Compartmentalization and actin binding properties of ABP-50: the elongation factor-1 alpha of Dictyostelium. Cell Motil Cytoskeleton. 1991; 20(4):279-88.
43. Yang F, Demma M, Warren V, Dharmawardhane S, Condeelis J. Identification of an actin-binding protein from Dictyostelium as elongation factor 1a. Nature. 1990 Oct 04; 347(6292):494-6.
44. Demma M, Warren V, Hock R, Dharmawardhane S, Condeelis J. Isolation of an abundant 50,000-dalton actin filament bundling protein from Dictyostelium amoebae. J Biol Chem. 1990 Feb 05; 265(4):2286-91.
45. Condeelis J, Bresnick A, Demma M, Dharmawardhane S, Eddy R, Hall AL, Sauterer R, Warren V. Mechanisms of amoeboid chemotaxis: an evaluation of the cortical expansion model. Dev Genet. 1990; 11(5-6):333-40.
46. Hall AL, Warren V, Dharmawardhane S, Condeelis J. Identification of actin nucleation activity and polymerization inhibitor in ameboid cells: their regulation by chemotactic stimulation. J Cell Biol. 1989 Nov; 109(5):2207-13.
47. Dharmawardhane S, Rubinstein B, Stern AI. Regulation of transplasmalemma electron transport in oat mesophyll cells by sphingoid bases and blue light. Plant Physiol. 1989 Apr; 89(4):1345-50.
48. Dharmawardhane S, Warren V, Hall AL, Condeelis J. Changes in the association of actin-binding proteins with the actin cytoskeleton during chemotactic stimulation of Dictyostelium discoideum. Cell Motil Cytoskeleton. 1989; 13(1):57-63.

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Breast Neoplasms
Stilbenes
Antineoplastic Agents
Pyrimidines
rac GTP-Binding Proteins
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