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Josiah Ochieng
Title Professor
Faculty Rank
Degree Ph.D.
Institution Meharry Medical College
Department Biochemistry and Cancer Biology
Clusters Cancer
Address
_1005 D.B. Todd Blvd.,
Nashville, TN 37208
City Nashville
State TN
Postal Code 37208
Telephone _615-327-6119
Fax
Email
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Title Adjunct Professor
Institution Vanderbilt University
Department Cancer Biology

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  My laboratory has investigated the potential role of fetuin-A (also known as alpha 2HS glycoprotein) in tumor progression for the past 20 years. In 2013, we demonstrated and reported that fetuin-A drives the progression of head and neck squamous cell carcinomas. More recently a number of studies have indicated that fetuin-A is up-regulated in a number of solid tumors including pancreatic, breast and prostate tumors. We have now demonstrated (published data) that fetuin-A is a major driver of growth and metastasis in glioblastomas. We will soon embark on pre-clinical studies using mouse models for glioblastomas. This study is of immense interest to us and we would like to engage as many collaborators as possible. Currently our focus is to determine the mechanisms by which fetuin-A modifies adhesion and growth competent exosomes during tumor growth and metastasis. I am also interested in looking at the role of fetuin-A in prostate cancer progression. This disease disproportionately affects men of African descent.

Mentorship icon

 
Availability Date:
Monday, February 12, 2018

My goal as a mentor is to inspire my mentees to imagine more. As Albert Einstein once said, "Imagination is more important than knowledge." I have always encouraged my mentees to think outside the "box" and ask questions that others do not want to ask. This can be risky but scientific reward if successful is tremendous!!

I am available to mentor as:
I am available to mentor junior faculty from RCMI schools.

Contact Information for Mentoring:
My e-mail address is jochieng@mmc.edu

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Programs, Membership, and Biomedical Research icon

Mentoring Mentor

NIH Awarded Grants icon

  Research projects funded by the National Institutes of Health (NIH), the Centers for Disease Control (CDC), the Food and Drug Administration (FDA), and the Department of Veterans Affairs (VA)

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1.
2015Analysis of Serum Exsomes in Patients with Colorectal and Breast Cancer5U54CA163069-05
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2014Analysis of Serum Exsomes in Patients with Colorectal and Breast Cancer5U54CA163069-04
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2013Analysis of Serum Exsomes in Patients with Colorectal and Breast Cancer5U54CA163069-03
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2012Role of Fetuin-A in Tumor Cell Growth3SC1CA134018-04S1
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2012Analysis of Serum Exsomes in Patients with Colorectal and Breast Cancer5U54CA163069-02
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2011Analysis of Serum Exsomes in Patients with Colorectal and Breast Cancer1U54CA163069-01
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2010Role of Fetuin-A in Tumor Cell Growth5SC1CA134018-04
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2009Role of Fetuin-A in Tumor Cell Growth5SC1CA134018-03
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2009Role of Fetuin-A in Tumor Cell Growth3SC1CA134018-03S1
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2008Role of Fetuin-A in Tumor Cell Growth5SC1CA134018-02
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2007Role of Fetuin-A in Tumor Cell Growth1SC1CA134018-01
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2002INTEGRIN ACTIVITIES DURING TUMOR PROGRESSION BY GALECTIN-35S06GM008037-31
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2002INTEGRIN ACTIVITIES DURING TUMOR PROGRESSION BY GALECTIN-33S06GM008037-30S3
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2002ROLES OF FETUIN IN TUMORIGENESIS AND METASTASIS5U54CA091408-02
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2002INTEGRIN ACTIVITIES DURING TUMOR PROGRESSION BY GALECTIN-33S06GM008037-31S1
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2002Pilot--Fetuin in tumorigenesis and metastasis5U54CA091405-02
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2002CORE--TISSUE CULTURE AND PROCUREMENT5U54CA091408-02
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2001INTEGRIN ACTIVITIES DURING TUMOR PROGRESSION BY GALECTIN-33S06GM008037-30S1
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2001INTEGRIN ACTIVITIES DURING TUMOR PROGRESSION BY GALECTIN-35S06GM008037-30
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2001ROLES OF FETUIN IN TUMORIGENESIS AND METASTASIS1U54CA091408-01
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2001INTEGRIN ACTIVITIES DURING TUMOR PROGRESSION BY GALECTIN-33S06GM008037-30S2
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2001Pilot--Fetuin in tumorigenesis and metastasis1U54CA091405-01
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2001CORE--TISSUE CULTURE AND PROCUREMENT1U54CA091408-01
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2000INTEGRIN ACTIVITIES DURING TUMOR PROGRESSION BY GALECTIN-35S06GM008037-29
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1999INTERACTIONS OF GELATINASES WITH SERUM/MEMBRANE PROTEIN5K14CA068281-05
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1999INTERACTIONS OF GELATINASES WITH SERUM /MEMBRANE PROTEIN3K14CA068281-04S1
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1999INTEGRIN ACTIVITIES DURING TUMOR PROGRESSION BY GALECTIN-32S06GM008037-28
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1998INTERACTIONS OF GELATINASES WITH SERUM/MEMBRANE PROTEIN5K14CA068281-04
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1997INTERACTIONS OF GELATINASES WITH SERUM/MEMBRANE PROTEIN5K14CA068281-03
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1996INTERACTIONS OF GELATINASES WITH SERUM/MEMBRANE PROTEIN5K14CA068281-02
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1995INTERACTIONS OF GELATINASES WITH SERUM/MEMBRANE PROTEIN1K14CA068281-01

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1. Ochieng J, Nangami G, Sakwe A, Rana T, Ingram S, Goodwin JS, Moye C, Lammers P, Adunyah SE. Extracellular histones are the ligands for the uptake of exosomes and hydroxyapatite-nanoparticles by tumor cells via syndecan-4. FEBS Lett. 2018 Sep 04.
2. Ochieng J, Nangami G, Sakwe A, Moye C, Alvarez J, Whalen D, Thomas P, Lammers P. Impact of Fetuin-A (AHSG) on Tumor Progression and Type 2 Diabetes. Int J Mol Sci. 2018 Jul 29; 19(8).
3. Wang L, Widatalla SE, Whalen DS, Ochieng J, Sakwe AM. Association of calcium sensing receptor polymorphisms at rs1801725 with circulating calcium in breast cancer patients. BMC Cancer. 2017 Aug 02; 17(1):511.
4. Nangami GN, Sakwe AM, Izban MG, Rana T, Lammers PE, Thomas P, Chen Z, Ochieng J. Fetuin-A (alpha 2HS glycoprotein) modulates growth, motility, invasion, and senescence in high-grade astrocytomas. Cancer Med. 2016 Dec; 5(12):3532-3543.
5. Higginbotham JN, Zhang Q, Jeppesen DK, Scott AM, Manning HC, Ochieng J, Franklin JL, Coffey RJ. Identification and characterization of EGF receptor in individual exosomes by fluorescence-activated vesicle sorting. J Extracell Vesicles. 2016; 5:29254.
6. Ochieng J, Nangami GN, Ogunkua O, Miousse IR, Koturbash I, Odero-Marah V, McCawley LJ, Nangia-Makker P, Ahmed N, Luqmani Y, Chen Z, Papagerakis S, Wolf GT, Dong C, Zhou BP, Brown DG, Colacci AM, Hamid RA, Mondello C, Raju J, Ryan EP, Woodrick J, Scovassi AI, Singh N, Vaccari M, Roy R, Forte S, Memeo L, Salem HK, Amedei A, Al-Temaimi R, Al-Mulla F, Bisson WH, Eltom SE. The impact of low-dose carcinogens and environmental disruptors on tissue invasion and metastasis. Carcinogenesis. 2015 Jun; 36 Suppl 1:S128-59.
7. Goodson WH, Lowe L, Carpenter DO, Gilbertson M, Manaf Ali A, Lopez de Cerain Salsamendi A, Lasfar A, Carnero A, Azqueta A, Amedei A, Charles AK, Collins AR, Ward A, Salzberg AC, Colacci A, Olsen AK, Berg A, Barclay BJ, Zhou BP, Blanco-Aparicio C, Baglole CJ, Dong C, Mondello C, Hsu CW, Naus CC, Yedjou C, Curran CS, Laird DW, Koch DC, Carlin DJ, Felsher DW, Roy D, Brown DG, Ratovitski E, Ryan EP, Corsini E, Rojas E, Moon EY, Laconi E, Marongiu F, Al-Mulla F, Chiaradonna F, Darroudi F, Martin FL, Van Schooten FJ, Goldberg GS, Wagemaker G, Nangami GN, Calaf GM, Williams G, Wolf GT, Koppen G, Brunborg G, Lyerly HK, Krishnan H, Ab Hamid H, Yasaei H, Sone H, Kondoh H, Salem HK, Hsu HY, Park HH, Koturbash I, Miousse IR, Scovassi AI, Klaunig JE, Vondrácek J, Raju J, Roman J, Wise JP, Whitfield JR, Woodrick J, Christopher JA, Ochieng J, Martinez-Leal JF, Weisz J, Kravchenko J, Sun J, Prudhomme KR, Narayanan KB, Cohen-Solal KA, Moorwood K, Gonzalez L, Soucek L, Jian L, D'Abronzo LS, Lin LT, Li L, Gulliver L, McCawley LJ, Memeo L, Vermeulen L, Leyns L, Zhang L, Valverde M, Khatami M, Romano MF, Chapellier M, Williams MA, Wade M, Manjili MH, Lleonart ME, Xia M, Gonzalez MJ, Karamouzis MV, Kirsch-Volders M, Vaccari M, Kuemmerle NB, Singh N, Cruickshanks N, Kleinstreuer N, van Larebeke N, Ahmed N, Ogunkua O, Krishnakumar PK, Vadgama P, Marignani PA, Ghosh PM, Ostrosky-Wegman P, Thompson PA, Dent P, Heneberg P, Darbre P, Sing Leung P, Nangia-Makker P, Cheng QS, Robey RB, Al-Temaimi R, Roy R, Andrade-Vieira R, Sinha RK, Mehta R, Vento R, Di Fiore R, Ponce-Cusi R, Dornetshuber-Fleiss R, Nahta R, Castellino RC, Palorini R, Abd Hamid R, Langie SA, Eltom SE, Brooks SA, Ryeom S, Wise SS, Bay SN, Harris SA, Papagerakis S, Romano S, Pavanello S, Eriksson S, Forte S, Casey SC, Luanpitpong S, Lee TJ, Otsuki T, Chen T, Massfelder T, Sanderson T, Guarnieri T, Hultman T, Dormoy V, Odero-Marah V, Sabbisetti V, Maguer-Satta V, Rathmell WK, Engström W, Decker WK, Bisson WH, Rojanasakul Y, Luqmani Y, Chen Z, Hu Z. Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: the challenge ahead. Carcinogenesis. 2015 Jun; 36 Suppl 1:S254-96.
8. Nangami G, Koumangoye R, Shawn Goodwin J, Sakwe AM, Marshall D, Higginbotham J, Ochieng J. Fetuin-A associates with histones intracellularly and shuttles them to exosomes to promote focal adhesion assembly resulting in rapid adhesion and spreading in breast carcinoma cells. Exp Cell Res. 2014 Nov 01; 328(2):388-400.
9. Koumangoye RB, Nangami GN, Thompson PD, Agboto VK, Ochieng J, Sakwe AM. Reduced annexin A6 expression promotes the degradation of activated epidermal growth factor receptor and sensitizes invasive breast cancer cells to EGFR-targeted tyrosine kinase inhibitors. Mol Cancer. 2013 Dec 19; 12(1):167.
10. Thompson PD, Sakwe A, Koumangoye R, Yarbrough WG, Ochieng J, Marshall DR. Alpha-2 Heremans Schmid Glycoprotein (AHSG) modulates signaling pathways in head and neck squamous cell carcinoma cell line SQ20B. Exp Cell Res. 2014 Feb 15; 321(2):123-32.
11. Nangami GN, Watson K, Parker-Johnson K, Okereke KO, Sakwe A, Thompson P, Frimpong N, Ochieng J. Fetuin-A (a2HS-glycoprotein) is a serum chemo-attractant that also promotes invasion of tumor cells through Matrigel. Biochem Biophys Res Commun. 2013 Sep 06; 438(4):660-5.
12. Johnson KP, Yearby LA, Stoute D, Burow ME, Rhodes LV, Gray M, Carriere P, Tilghman SL, McLachlan JA, Ochieng J. In vitro and in vivo evaluation of novel anticancer agents in triple negative breast cancer models. J Health Care Poor Underserved. 2013 Feb; 24(1 Suppl):104-11.
13. Watson K, Koumangoye R, Thompson P, Sakwe AM, Patel T, Pratap S, Ochieng J. Fetuin-A triggers the secretion of a novel set of exosomes in detached tumor cells that mediate their adhesion and spreading. FEBS Lett. 2012 Sep 21; 586(19):3458-63.
14. Koumangoye RB, Sakwe AM, Goodwin JS, Patel T, Ochieng J. Detachment of breast tumor cells induces rapid secretion of exosomes which subsequently mediate cellular adhesion and spreading. PLoS One. 2011; 6(9):e24234.
15. Sakwe AM, Koumangoye R, Guillory B, Ochieng J. Annexin A6 contributes to the invasiveness of breast carcinoma cells by influencing the organization and localization of functional focal adhesions. Exp Cell Res. 2011 Apr 01; 317(6):823-37.
16. Sakwe AM, Koumangoye R, Goodwin SJ, Ochieng J. Fetuin-A ({alpha}2HS-glycoprotein) is a major serum adhesive protein that mediates growth signaling in breast tumor cells. J Biol Chem. 2010 Dec 31; 285(53):41827-35.
17. Guillory B, Sakwe AM, Saria M, Thompson P, Adhiambo C, Koumangoye R, Ballard B, Binhazim A, Cone C, Jahanen-Dechent W, Ochieng J. Lack of fetuin-A (alpha2-HS-glycoprotein) reduces mammary tumor incidence and prolongs tumor latency via the transforming growth factor-beta signaling pathway in a mouse model of breast cancer. Am J Pathol. 2010 Nov; 177(5):2635-44.
18. Ochieng J, Chaudhuri G. Cystatin superfamily. J Health Care Poor Underserved. 2010 Feb; 21(1 Suppl):51-70.
19. Ochieng J, Pratap S, Khatua AK, Sakwe AM. Anchorage-independent growth of breast carcinoma cells is mediated by serum exosomes. Exp Cell Res. 2009 Jul 01; 315(11):1875-88.
20. Baptiste TA, James A, Saria M, Ochieng J. Mechano-transduction mediated secretion and uptake of galectin-3 in breast carcinoma cells: implications in the extracellular functions of the lectin. Exp Cell Res. 2007 Feb 15; 313(4):652-64.
21. Lukyanov P, Furtak V, Ochieng J. Galectin-3 interacts with membrane lipids and penetrates the lipid bilayer. Biochem Biophys Res Commun. 2005 Dec 16; 338(2):1031-6.
22. Kundranda MN, Henderson M, Carter KJ, Gorden L, Binhazim A, Ray S, Baptiste T, Shokrani M, Leite-Browning ML, Jahnen-Dechent W, Matrisian LM, Ochieng J. The serum glycoprotein fetuin-A promotes Lewis lung carcinoma tumorigenesis via adhesive-dependent and adhesive-independent mechanisms. Cancer Res. 2005 Jan 15; 65(2):499-506.
23. Kleshchenko YY, Moody TN, Furtak VA, Ochieng J, Lima MF, Villalta F. Human galectin-3 promotes Trypanosoma cruzi adhesion to human coronary artery smooth muscle cells. Infect Immun. 2004 Nov; 72(11):6717-21.
24. Kundranda MN, Ray S, Saria M, Friedman D, Matrisian LM, Lukyanov P, Ochieng J. Annexins expressed on the cell surface serve as receptors for adhesion to immobilized fetuin-A. Biochim Biophys Acta. 2004 Aug 23; 1693(2):111-23.
25. Leite-Browning ML, McCawley LJ, Jahnen-Dechent W, King LE, Matrisian LM, Ochieng J. Alpha 2-HS glycoprotein (fetuin-A) modulates murine skin tumorigenesis. Int J Oncol. 2004 Aug; 25(2):319-24.
26. Ray S, Lukyanov P, Ochieng J. Members of the cystatin superfamily interact with MMP-9 and protect it from autolytic degradation without affecting its gelatinolytic activities. Biochim Biophys Acta. 2003 Dec 01; 1652(2):91-102.
27. Leite-Browning ML, McCawley LJ, Choi OH, Matrisian LM, Ochieng J. Interactions of alpha2-HS-glycoprotein (fetuin) with MMP-3 and murine squamous cell carcinoma cells. Int J Oncol. 2002 Nov; 21(5):965-71.
28. Ochieng J, Furtak V, Lukyanov P. Extracellular functions of galectin-3. Glycoconj J. 2002; 19(7-9):527-35.
29. Furtak V, Hatcher F, Ochieng J. Galectin-3 mediates the endocytosis of beta-1 integrins by breast carcinoma cells. Biochem Biophys Res Commun. 2001 Dec 14; 289(4):845-50.
30. Zhu WQ, Ochieng J. Rapid release of intracellular galectin-3 from breast carcinoma cells by fetuin. Cancer Res. 2001 Mar 01; 61(5):1869-73.
31. Moody TN, Ochieng J, Villalta F. Novel mechanism that Trypanosoma cruzi uses to adhere to the extracellular matrix mediated by human galectin-3. FEBS Lett. 2000 Mar 31; 470(3):305-8.
32. Ochieng J, Warfield P, Green-Jarvis B, Fentie I. Galectin-3 regulates the adhesive interaction between breast carcinoma cells and elastin. J Cell Biochem. 1999 Dec 01; 75(3):505-14.
33. Ochieng J, Leite-Browning ML, Warfield P. Regulation of cellular adhesion to extracellular matrix proteins by galectin-3. Biochem Biophys Res Commun. 1998 May 29; 246(3):788-91.
34. Ochieng J, Green B, Evans S, James O, Warfield P. Modulation of the biological functions of galectin-3 by matrix metalloproteinases. Biochim Biophys Acta. 1998 Jan 08; 1379(1):97-106.
35. Ochieng J, Warfield P, Johnson KN. Establishment of breast cell cultures and lines from peoples of African origin. Am J Obstet Gynecol. 1997 Jun; 176(6):S240-5.
36. Warfield PR, Makker PN, Raz A, Ochieng J. Adhesion of human breast carcinoma to extracellular matrix proteins is modulated by galectin-3. Invasion Metastasis. 1997; 17(2):101-12.
37. Ochieng J, Green B. The interactions of alpha 2HS glycoprotein with metalloproteinases. Biochem Mol Biol Int. 1996 Sep; 40(1):13-20.
38. Johnson DE, Ochieng J, Evans SL. Phenylacetic acid halides inhibit estrogen receptor (ER)-positive MCF-7 cells, but not ER-negative human breast cancer cells or normal breast epithelial cells. Anticancer Drugs. 1996 May; 7(3):288-92.
39. Ochieng J, Warfield P. Galectin-3 binding potentials of mouse tumor EHS and human placental laminins. Biochem Biophys Res Commun. 1995 Dec 14; 217(2):402-6.
40. Nangiamakker P, Thompson E, Hogan C, Ochieng J, Raz A. Induction of tumorigenicity by galectin-3 in a nontumorigenic human breast-carcinoma cell-line. Int J Oncol. 1995 Nov; 7(5):1079-87.
41. Ochieng J, Warfield P, Green B. Interactions of gelatinases with soluble and immobilized fetuin and asialofetuin. Arch Biochem Biophys. 1995 Sep 10; 322(1):250-5.
42. Johnson DE, Ochieng J, Evans SL. The growth inhibitory properties of a dopamine agonist (SKF 38393) on MCF-7 cells. Anticancer Drugs. 1995 Jun; 6(3):471-4.
43. Ochieng J, Fridman R, Nangia-Makker P, Kleiner DE, Liotta LA, Stetler-Stevenson WG, Raz A. Galectin-3 is a novel substrate for human matrix metalloproteinases-2 and -9. Biochemistry. 1994 Nov 29; 33(47):14109-14.
44. Nangia-Makker P, Ochieng J, Christman JK, Raz A. Regulation of the expression of galactoside-binding lectin during human monocytic differentiation. Cancer Res. 1993 Oct 15; 53(20):5033-7.
45. Ochieng J, Gerold M, Raz A. Dichotomy in the laminin-binding properties of soluble and membrane-bound human galactoside-binding protein. Biochem Biophys Res Commun. 1992 Aug 14; 186(3):1674-80.
46. Ochieng J, Tahin QS, Booth CC, Russo J. Buffering of intracellular calcium in response to increased extracellular levels in mortal, immortal, and transformed human breast epithelial cells. J Cell Biochem. 1991 Jul; 46(3):250-4.
47. Ochieng J, Basolo F, Albini A, Melchiori A, Watanabe H, Elliott J, Raz A, Parodi S, Russo J. Increased invasive, chemotactic and locomotive abilities of c-Ha-ras-transformed human breast epithelial cells. Invasion Metastasis. 1991; 11(1):38-47.
48. Ochieng J, Tait L, Russo J. Calcium-mediated modulation of microtubule assembly in human breast epithelial cells. In Vitro Cell Dev Biol. 1990 Apr; 26(4):318-24.
49. Muralidhar G, Ochieng J, Trewyn RW. Altered queuine modification of transfer RNA involved in the in vitro transformation of Chinese hamster embryo cells. Cancer Res. 1989 Dec 15; 49(24 Pt 1):7110-4.
50. Ochieng J, Patrick DE, Utz ED, Trewyn RW. Inhibition of phorbol ester-mediated phenotypic changes in cultured cells by hypoxanthine. Carcinogenesis. 1987 Nov; 8(11):1629-33.

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Keywords
Breast Neoplasms
alpha-2-HS-Glycoprotein
Cell Adhesion
Exosomes
Humans
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