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Margaret Baker, PhD

TitleProteomics Core Co-Director
Faculty RankJunior Researcher
InstitutionUniversity of Hawaii
DepartmentMolecular Biosciences & Bioengineering
AddressUniversity of Hawaii
1955 East-West Road
Suite 218
Honolulu HI 96822
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    Collapse Biography 
    Collapse awards and honors
    2011Helen Jones Farrar Award in Tropical Agriculture, Achievement Rewards for College Scientists, University of Hawaii
    2012 - 2014Pre-doctoral Fellowship, National Institute of Food and Agriculture

    Collapse Overview 
    Collapse overview
    Dr. Margaret Baker serves as the University of Hawaii, John A. Burns School of Medicine Proteomics Core Co-Director. She maintains three mass spectrometers, provides consultation for the medical community and other departments, and conducts proteomics experiments.

    During her predoctoral work, she explored the role of N-glycosylation in a stable peroxidase from palm tree leaves. Plant peroxidases are extensively used in bioresearch and bioanalysis. These enzymes are also capable of synthesizing conductive, biodegradable plastics, and remediation of contaminated industrial wastewater. Plant peroxidases rely on N-glycosylation for full enzyme activity and stability, however, the mechanism is poorly understood. She developed a sensitive technique for mass spectrometry-based amino acid sequencing, published in Analytical Chemistry. Next, she used a mass spectrometry-guided approach to clone the gene and verify the amino acid sequence. They found that windmill palm tree peroxidase is a heterogeneous glycoprotein with 13 sites for N-glycosylation. Next, she studied the site-specific glycosylation of windmill palm tree peroxidase, which was published in Journal of Proteome Research. In this work, she overcame the lack of tools for interpreting plant glycopeptide mass spectrometry data by developing a workflow and bioinformatics tools. The analysis gave an in-depth view of the glycosylation of windmill palm tree peroxidase.

    Her current research examines the contribution of native glycosylation to windmill palm tree peroxidase function by comparing the native enzyme to those expressed in Nicotiana benthamiana and Pichia pastoris. Differences in glycosylation are hypothesized to affect enzyme stability and activity. Her workflow and tools for analysis of plant glycopeptide mass spectrometry data will be instrumental for interpretation of the results.

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Seiber JN, Baker MR, Li QX. Protecting and Enhancing Scarce Water Resources through Chemistry. J Agric Food Chem. 2021 Aug 06. PMID: 34355570.
      Citations:    Fields:    
    2. Sun B, Tan D, Pan D, Baker MR, Liang Z, Wang Z, Lei J, Liu S, Hu CY, Li QX. Dihydromyricetin Imbues Antiadipogenic Effects on 3T3-L1 Cells via Direct Interactions with 78-kDa Glucose-Regulated Protein. J Nutr. 2021 Jul 01; 151(7):1717-1725. PMID: 33830233.
      Citations: 1     Fields:    
    3. An F, Baker MR, Qin Y, Chen S, Li QX. Relevance of Class I a-Mannosidases to Cassava Postharvest Physiological Deterioration. ACS Omega. 2019 May 31; 4(5):8739-8746. PMID: 31459963.
    4. Fu B, Baker MR, Li QX. Effect of N-Linked Glycosylation of Recombinant Windmill Palm Tree Peroxidase on Its Activity and Stability. J Agric Food Chem. 2018 May 02; 66(17):4414-4421. PMID: 29648454.
      Citations:    Fields:    Translation:AnimalsCells
    5. Chu S, Baker MR, Leong G, Letcher RJ, Li QX. Covalent binding of the organophosphate insecticide profenofos to tyrosine on a- and ß-tubulin proteins. Chemosphere. 2018 May; 199:154-159. PMID: 29433029.
      Citations: 1     Fields:    Translation:AnimalsCells
    6. Baker MR, Ching T, Tabb DL, Li QX. Characterization of Plant Glycoproteins: Analysis of Plant Glycopeptide Mass Spectrometry Data with plantGlycoMS, a Package in the R Statistical Computing Environment. Methods Mol Biol. 2018; 1789:205-220. PMID: 29916082.
      Citations:    Fields:    Translation:AnimalsCells
    7. Wen B, Baker MR, Zhao H, Cui Z, Li QX. Expression and Characterization of Windmill Palm Tree (Trachycarpus fortunei) Peroxidase by Pichia pastoris. J Agric Food Chem. 2017 Jun 14; 65(23):4676-4682. PMID: 28523913.
      Citations:    Fields:    Translation:AnimalsCells
    8. Chu S, Baker MR, Leong G, Letcher RJ, Gee SJ, Hammock BD, Li QX. Exploring adduct formation between human serum albumin and eleven organophosphate ester flame retardants and plasticizers using MALDI-TOF/TOF and LC-Q/TOF. Chemosphere. 2017 Aug; 180:169-177. PMID: 28407546.
      Citations: 3     Fields:    Translation:HumansCells
    9. Baker MR, Tabb DL, Ching T, Zimmerman LJ, Sakharov IY, Li QX. Site-Specific N-Glycosylation Characterization of Windmill Palm Tree Peroxidase Using Novel Tools for Analysis of Plant Glycopeptide Mass Spectrometry Data. J Proteome Res. 2016 06 03; 15(6):2026-38. PMID: 27151270.
      Citations: 3     Fields:    Translation:AnimalsCells
    10. Baker MR, Zhao H, Sakharov IY, Li QX. Amino acid sequence of anionic peroxidase from the windmill palm tree Trachycarpus fortunei. J Agric Food Chem. 2014 Dec 10; 62(49):11941-8. PMID: 25383699.
      Citations:    Fields:    Translation:AnimalsCells
    11. Bergeron ZL, Chun JB, Baker MR, Sandall DW, Peigneur S, Yu PY, Thapa P, Milisen JW, Tytgat J, Livett BG, Bingham JP. A 'conovenomic' analysis of the milked venom from the mollusk-hunting cone snail Conus textile--the pharmacological importance of post-translational modifications. Peptides. 2013 Nov; 49:145-58. PMID: 24055806.
      Citations: 4     Fields:    Translation:AnimalsCells
    12. Baker MR, Li QX. Guanidination of tryptic peptides without desalting for matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry analysis. Anal Chem. 2013 Sep 17; 85(18):8873-80. PMID: 23964694.
      Citations: 1     Fields:    Translation:Animals
    13. Bingham JP, Baker MR, Chun JB. Analysis of a cone snail's killer cocktail--the milked venom of Conus geographus. Toxicon. 2012 Nov; 60(6):1166-70. PMID: 22884604.
      Citations: 5     Fields:    Translation:AnimalsCells
    14. Chun JB, Baker MR, Kim DH, Leroy M, Toribo P, Bingham JP. Cone snail milked venom dynamics--a quantitative study of Conus purpurascens. Toxicon. 2012 Jul; 60(1):83-94. PMID: 22497788.
      Citations: 10     Fields:    Translation:AnimalsCells
    15. Bingham JP, Chun JB, Ruzicka MR, Li QX, Tan ZY, Kaulin YA, Englebretsen DR, Moczydlowski EG. Synthesis of an iberiotoxin derivative by chemical ligation: a method for improved yields of cysteine-rich scorpion toxin peptides. Peptides. 2009 Jun; 30(6):1049-57. PMID: 19463736.
      Citations: 3     Fields:    Translation:HumansCells
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